食品科学

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ELISA和UPLC-MS/MS联合检测粮食中玉米赤霉烯酮残留

李 妍,裴世春,王 岩,高建伟,甄玉萍,马明欣,张 品,谢铁志   

  1. 1.齐齐哈尔大学食品与生物工程学院,黑龙江 齐齐哈尔 161006;
    2.岛津企业管理(中国)有限公司沈阳分公司,辽宁 沈阳 110016;
    3.泰来县绿洲食品加工有限责任公司,黑龙江 齐齐哈尔 162400
  • 出版日期:2016-08-25 发布日期:2016-08-30
  • 通讯作者: 裴世春
  • 基金资助:

    国家科技基础性工作专项(2013FY113400);黑龙江省教育厅科学计划项目(12541871)

Combined Use of ELISA and UPLC-MS/MS for Detection of Zearalenone from Grains

LI Yan, PEI Shichun, WANG Yan, GAO Jianwei, ZHEN Yuping, MA Mingxin, ZHANG Pin, XIE Tiezhi   

  1. 1. College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China;
    2. Shenyang Analytical Application Center, Shimadzu (China) Co. Ltd., Shenyang 110016, China;
    3. Tailai Oasis Food Processing Co. Ltd., Qiqihar 162400, China
  • Online:2016-08-25 Published:2016-08-30
  • Contact: 裴世春

摘要:

建立酶联免疫吸附分析(enzyme-linked immunosorbent assay,ELISA)和超高效液相色谱-串联质谱(ultraperformance liquid chromatograph-tandem mass spectrometry,UPLC-MS/MS)联合测定粮食中玉米赤霉烯酮的快速检测方法。样品经乙腈-水-乙酸(70∶29∶1,V/V)溶液提取,采用ELISA筛选出阳性样品后利用UPLC-MS/MS进行验证。实验探讨了ELISA和UPLC-MS/MS联合检测法的有效性和测定中存在的问题及其矫正方法,并且研究了UPLC-MS/MS中样品净化与未净化的回收率。结果表明,本实验建立的ELISA和UPLC-MS/MS联合检测方法快速、高效,适用于大批量粮食样品中玉米赤霉烯酮残留量的快速评估。

关键词: 玉米赤霉烯酮, 酶联免疫分析法, 超高效液相色谱-串联质谱联用仪, 粮食, 检测

Abstract:

An enzyme-linked immunosorbent assay (ELISA) coupled to ultra performance liquid chromatography-tandem
mass spectrometry (UPLC-MS/MS) method was developed for the determination of zearalenone in corn samples. Samples
were extracted with acetonitrile-water-acetic acid (70:29:1, V/V) and then the extract was determined by ELISA and UPLCMS/
MS. In the study, special attention was given to the stability of the system and the matrix effects frequently observed in
the process. The recovery of UPLC-MS/MS with and without sample purification was evaluated as well. Proper approaches
for dealing with these problems were obtained. This analysis method proved easy, fast and suitable for the determination of
zearalenone in bulk grain samples.

Key words: zearalenone, ELISA, UPLC-MS/MS, grain, detection

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