磁性Fe3O4纳米带鱼肽微粒的制备及对CW-2细胞膜流动性的影响

doi:10.7506/spkx1002-6630-201619005

摘要/Abstract

摘要：

目的：制备磁性Fe3O4纳米带鱼肽微粒，并研究其对CW-2细胞膜流动性的影响。方法：以磁性Fe3O4纳米微粒为内核，负载具有抑制肿瘤增殖作用的带鱼酶解小肽，通过共沉淀法合成磁性Fe3O4纳米带鱼肽微粒，采用X射线衍射、透射式电子显微镜、原子力显微镜等方法对该纳米粒子结构进行表征；利用荧光偏振法研究该微粒在非磁场与交变磁场中对CW-2人结肠癌细胞膜流动性的影响。结果：共沉淀法合成的磁性Fe3O4纳米带鱼肽微粒呈球形，粒径约10 nm，分布较均匀，颗粒之间有黏连现象，形成缠绕弯曲的线状。与单体磁性Fe3O4纳米微粒相比，带鱼酶解小肽的包覆增强了纳米铁微粒的分散稳定性；该粒子最佳使用pH值范围是6.5～9.0，比较适合于在生物体系中应用。细胞膜流动性检测显示24 h时实验组CW-2细胞膜荧光偏振度P值显著减小、平均微黏度η值减小，表明磁性Fe3O4纳米带鱼肽微粒可使CW-2细胞膜流动性增大，作用呈量效关系。结论：磁性Fe3O4纳米带鱼肽微粒在交变磁场中增强了带鱼酶解小肽的抗肿瘤活性。

关键词: 磁性Fe3O4纳米微粒, 带鱼酶解小肽, 结构表征, CW-2细胞, 细胞膜流动性

Abstract:

Objective: To prepare magnetic Fe3O4 nanoparticles loading peptides derived from enzymatic hydrolysis of
hairtail processing waste proteins and explore its effect on membrane fluidity in CW-2 cells. Methods: The target product
was synthesized by co-precipitation method, and its structure characteristics and particle size distribution were observed with
X-ray diffraction (XRD), transmission electron microscope (TEM) and atomic force microscope (AFM). Meanwhile, its effect
on membrane fluidity in human colon cancer CW-2 cells was explored by fluorescence polarization in a non-magnetic field
and an alternating magnetic field. Results: Magnetic Fe3O4 nanoparticles loading hairtail peptides were uniformly distributed
with a size of approximately 10 nm and they were found to adhere to each other to form intertwined curves. Compared
with the single magnetic Fe3O4 particles, loading of hairtail peptides enhanced dispersion stability of magnetic nano-Fe3O4
particles. The optimum pH for magnetic Fe3O4 nanoparticles loading hairtail peptides was 6.5–9.0. Cell membrane fluidity
tests showed that P and η values in the experimental group were significantly reduced after 24 h of alternating magnetic field
exposure, indicating that the magnetic Fe3O4 nanoparticles loading hairtail peptides could increase cell membrane fluidity.
Conclusion: Magnetic Fe3O4 nanoparticles loading hairtail peptides can enhance the anti-tumor activity of hairtail peptides in
an alternating magnetic field.

Key words: magnetic Fe3O4 nanoparticles, hairtail enzymatic peptides, structure characterization, CW-2 cell, cell membrane fluidity

中图分类号:

石芸洁，林慧敏，金图南，邓尚贵. 磁性Fe3O4纳米带鱼肽微粒的制备及对CW-2细胞膜流动性的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201619005.

SHI Yunjie, LIN Huimin, JIN Tunan, DENG Shanggui. Preparation of Magnetic Fe3O4 Nanoparticles Loading Peptides Derived from Enzymatic Hydrolysis of Hairtail Waste Proteins and Its Effect on Membrane Fluidity in CW-2 Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201619005.

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磁性Fe3O4纳米带鱼肽微粒的制备及对CW-2细胞膜流动性的影响

Preparation of Magnetic Fe3O4 Nanoparticles Loading Peptides Derived from Enzymatic Hydrolysis of Hairtail Waste Proteins and Its Effect on Membrane Fluidity in CW-2 Cells

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