食品科学

• 基础研究 • 上一篇    下一篇

南瓜硒多糖的制备表征及活性分析

商龙臣1,吴少魏1,张 驰2,廖红华2,刘信平1,*   

  1. 1.湖北民族学院化学与环境工程学院,湖北 恩施 445000;2.湖北民族学院生物科学与技术学院,湖北 恩施 445000
  • 出版日期:2016-10-15 发布日期:2016-12-01

Preparation, Characterization and Activity Analysis of Selenium-Containing Pumpkin Polysaccharide

SHANG Longchen1, WU Shaowei1, ZHANG Chi2, LIAO Honghua2, LIU Xinping1,*   

  1. 1. College of Chemistry and Environmental Engineering, Hubei University for Nationalities, Enshi 445000, China;
    2. College of Biological Science and Technology, Hubei University for Nationalities, Enshi 445000, China
  • Online:2016-10-15 Published:2016-12-01

摘要:

对南瓜多糖进行硒化修饰,并对南瓜硒多糖的体外抗氧化和抑制人乳腺癌细胞MDA-MB-231生长等活性进行研究。以提取、分离、纯化的南瓜多糖为前体物,用Na2SeO3硒化修饰制备南瓜硒多糖,并用紫外光谱、红外光谱、原子荧光光谱、热重分析对产物结构进行表征,采用邻苯三酚自氧化法、水杨酸法、四甲基偶氮唑蓝比色法测定其清除超氧阴离子自由基(O2-•)、羟自由基(•OH)的能力以及对人乳腺癌细胞MDA-MB-231生长的抑制作用。结果表明:制备产物结构中含有Se=O键和Se—C键,即实现了南瓜多糖的硒化。南瓜硒多糖对O2 - ·、·OH的清除作用显著强于南瓜多糖,与样品量呈正相关;南瓜硒多糖对人乳腺癌细胞MDA-MB-231生长有抑制作用,比南瓜多糖具有更好的抑制效果。

关键词: 南瓜硒多糖, 制备, 表征, 活性

Abstract:

Objective: To investigate selenium modification of pumpkin polysaccharide and to evaluate the in vitro
antioxidant activity and antitumor effect against human breast cancer MDA-MB-231 cells of the modified polysaccharide.
Methods: Pumpkin polysaccharide was prepared and purified and it was modified by reaction with Na2SeO3 to obtain
selenium-containing polysaccharide. Furthermore, the structure of the modified polysaccharide was characterized through
UV, IR, atomic fluorescence spectroscopies and thermogravimetric analysis. Finally, its scavenging effect on O2-· and
·OH as well as growth inhibitory effect on breast cancer cells were tested by using pyrogallol autoxidation, salicylic
acid method and methyl thiazolyl tetrazolium colorimetric assay. Results: Se=O and Se–C bonds were observed in the
structure of the target product, thus successfully achieving the selenium modification of pumpkin polysaccharide. The
selenium-containing pumpkin polysaccharide had significantly stronger scavenging effects on O2-· and ·OH than ordinary
pumpkin polysaccharide, which were concentration-dependent. Meanwhile, the modified polysaccharide also inhibited the
growth of human breast cancer cells in a dose-dependent manner more significantly than did the unmodified one.

Key words: selenium-containing pumpkin polysaccharide, preparation, characterization, activity

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