食品科学

• 基础研究 • 上一篇    下一篇

苹果发育过程中细胞壁代谢及果肉质地的变化

高滋艺1,范献光1,杨惠娟1,蒋小兵1,杨亚州1,赵政阳1,2,*,党智宏3   

  1. 1.西北农林科技大学园艺学院,陕西 杨凌 712100;2.陕西省苹果工程技术研究中心,陕西 杨凌 712100;
    3.陕西省白水县园艺站,陕西 白水 715600
  • 出版日期:2016-10-15 发布日期:2016-12-01

Correlation among Cell Wall Components, Related Enzyme Activities and Texture of Developing Fruits of Different Apple (Malus × domestica) Cultivars

GAO Ziyi1, FAN Xianguang1, YANG Huijuan1, JIANG Xiaobing1, YANG Yazhou1, ZHAO Zhengyang1,2,*, DANG Zhihong3   

  1. 1. College of Horticulture, Northwest A&F University, Yangling 712100, China; 2. Apple E&T Research Centre of Shaanxi Province, Yangling 712100, China; 3. Baishui Horticultural Station of Shaanxi Province, Baishui 715600, China
  • Online:2016-10-15 Published:2016-12-01

摘要:

以质地存在差异的“蜜脆”、“嘎拉”、“倭锦”、“鸡冠”4 个品种的苹果为材料,分析了果实发育过程中硬度、脆度和单果质量的变化,以及细胞壁中果胶、纤维素、半纤维素的含量和多聚半乳糖醛酸酶(polygalacronase,PG)、果胶甲酯酶(pectinmethylesterase,PME)、纤维素酶(carboxymethycellulase,Cx)、β-半乳糖苷酶(β-galactosidase,β-Gal)活性的变化,以期找到品种间质地差异的主要因素。结果表明:果实发育过程中果实硬度降低、脆度变小、感官脆度增大,细胞壁各个组分的含量在4 个品种中均呈下降趋势;在成熟果实中,脆性较好的品种果实中纤维素含量较低,水溶性果胶(water soluble pectin,WSP)含量较高,硬度低的品种果实中离子结合果胶(ionic soluble pectin,ISP)含量较高;PG、β-Gal在高脆品种“蜜脆”、“嘎拉”中活性较高,而Cx、PME活力在各品种间无明显差异。认为WSP、ISP和纤维素含量的差异以及PG和β-Gal活性的差异是形成苹果果实质地差异的关键因素。

关键词: 苹果, 质地, 细胞壁, 脆度, 硬度

Abstract:

Apple texture is one of the most important quality properties that influences consumers’ acceptability of this
fruit. To evaluate the determinant factor for texture difference in apple fruits, the changes in firmness, crispness, cell wall
components and related enzyme activities in fruits of 4 apple cultivars with different textures were monitored during
fruit growth and ripening in this study. The results suggested that fruit crispness slightly increased whil firmness and the
contents of cell wall components in the 4 apple cultivars showed a decreasing tendency during fruit development. During
fruit ripening, the content of water-soluble pectin (WSP) in the crisp fruit cultivars was higher, while cellulose content
was obviously lower. The activities of polygalacronase (PG) and β-galactosidase were higher in the apple cultivars with
higher crispness ‘Honeycrisp’ and ‘Gala’, but the activities of carboxymethycellulase and pectinmethylesterase disclosed no
significant difference among all cultivars. Therefore, it was proposed that apple texture differed greatly due to variations in
WSP, ionic soluble pectin and cellulose contents as well as PG and β-galactosidase activities.

Key words: apple, texture, cell wall components, crispness, firmness

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