食品科学

• 基础研究 • 上一篇    下一篇

模拟体外消化对蓝靛果提取物花色苷组成及抗氧化能力的影响

王月华1,李 斌1,孟宪军1,*,安小琦1,马 岩2,张 琦1,李 丽1,李冬男1   

  1. 1.沈阳农业大学食品学院,辽宁 沈阳 110866;2.沈阳师范大学实验教学中心,辽宁 沈阳 110034
  • 出版日期:2016-10-15 发布日期:2016-12-01

Effect of in Vitro Simulated Digestion on Anthocyanin Composition and Antioxidant Activity of Lonicera caerulea Berry Extracts

WANG Yuehua1, LI Bin1, MENG Xianjun1,*, AN Xiaoqi1, MA Yan2, ZHANG Qi1, LI Li1, LI Dongnan1   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
    2. Experimental Teaching Center, Shenyang Normal University, Shenyang 110034, China
  • Online:2016-10-15 Published:2016-12-01

摘要:

研究体外消化前后蓝靛果提取物的总酚和花色苷含量、花色苷组成以及抗氧化能力的变化。实验分别采用福林-酚法和pH示差法测定总酚和花色苷含量,利用高效液相色谱-电喷雾二级质谱联用技术结合分子质量,离子碎片,出峰顺序及参考文献分析花色苷组成;采用总抗氧化能力(total antioxidant capacity,T-AOC)和氧自由基吸收能力(oxygen radical absorbance capacity,ORAC)法测定体外消化前后提取物的抗氧化能力。结果表明:模拟体外消化后样品总酚含量增加了48.2%,花色苷含量降低了67.6%,单体花色苷由11 种降为8 种,此外,ORAC值和T-AOC值分别下降了80.0%和55.6%。综上,与未经消化提取物相比较,模拟体外消化处理的蓝靛果提取物的多酚含量增加,花色苷含量和种类均减少,抗氧化能力下降。

关键词: 蓝靛果, 总酚含量, 花色苷组成, 氧自由基吸收能力, 总抗氧化能

Abstract:

Changes in total polyphenols and anthocyanins contents, anthocyanin composition and antioxidant capacity of
Lonicera caerulea berry extracts before and after in vitro digestion were investigated. Folin-Ciocalteu and pH differential
methods were used for determining the contents of total polyphenols and anthocyanins, respectively. Anthocyanin
composition was analyzed using HPLC-EIS-MS/MS based on molecular mass and fragment ions, peak sequence and related
literature data. Antioxidant activities of the extracts before and after in vitro digestion were measured using total antioxidant
capacity (T-AOC) and oxygen radical absorbance capacity (ORAC) methods. The results indicated that the content of total
polyphenols increased by 48.2% while anthocyanins decreased by 67.6%. The number of anthocyanins decreased from
11 to 8; additionally, the ORAC and T-AOC values of the extracts decreased by 80.0% and 55.6% after in vitro digestion,
respectively. Taken together, after in vitro digestion, Lonicera caerulea berry extracts had increased total polyphenols,
reduced anthocyanin contents and composition, and lowered antioxidant activity.

Key words: Lonicera caerulea, total polyphenol content, anthocyanin composition, oxygen radical absorbance capacity, total antioxidant capacity

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