食品科学

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NaCl胁迫下Ca2+调控发芽大豆生理代谢和γ-氨基丁酸含量变化

尹永祺,王淑雯,宋吴昱,高 璐,饶胜其,杨振泉,方维明*   

  1. 扬州大学食品科学与工程学院,江苏 扬州 225127
  • 出版日期:2016-11-15 发布日期:2016-11-18

Calcium Regulates the Main Physiological Indicators and Gamma-Aminobutyric Acid Accumulation in Germinating Soybean under NaCl Stress

YIN Yongqi, WANG Shuwen, SONG Wuyu, GAO Lu, RAO Shengqi, YANG Zhenquan, FANG Weiming*   

  1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China
  • Online:2016-11-15 Published:2016-11-18

摘要:

为研究NaCl胁迫下Ca2+对发芽大豆主要生理指标和γ-氨基丁酸(gamma-aminobutyric acid,GABA)富集的调控作用,利用CaCl2和乙二醇二乙醚二胺四乙酸(ethylene glycol tetraacetic acid,EGTA)处理发芽大豆,研究NaCl胁迫下外源和内源Ca2+对发芽大豆主要生理代谢和GABA含量的影响。结果显示,发芽大豆经NaCl联合CaCl2处理,其芽长和呼吸速率显著增加,表明CaCl2缓解了NaCl对发芽大豆生长的抑制,同时过氧化氢酶及过氧化物酶活力显著提高,说明CaCl2可能是通过提高抗氧化酶活力来缓解NaCl胁迫下发芽大豆的抑制效应,而施用EGTA则呈相反的变化趋势;NaCl联合CaCl2处理后发芽大豆中GABA含量与单独NaCl处理无显著差异,但显著高于对照组;在NaCl联合CaCl2或EGTA基础上施用氨基胍,发芽大豆子叶中GABA含量分别下降17.1%和9.8%,胚中分别下降26.5%和8.5%,表明NaCl胁迫下施用CaCl2在促进发芽大豆中GABA富集的同时还可保证生物产量,且CaCl2和EGTA处理下多胺降解途径对GABA富集贡献降低。

关键词: 大豆, 发芽, &gamma, -氨基丁酸, NaCl胁迫

Abstract:

In the present study, the effect of CaCl2 and ethylene glycol tetraacetic acid (EGTA) on the main physiological
indicators and gamma-aminobutyric acid (GABA) accumulation in germinating soybean under NaCl stress was investigated.
Compared with NaCl stress alone, the sprout length and respiratory rate increased significantly under NaCl stress combined
with CaCl2 treatment, indicating that exogenous CaCl2 mitigated the negative effect of NaCl stress. Meanwhile, CaCl2 led
to a significant increase in catalase (CAT) and peroxidase (POD) activities and could therefore alleviate the harmful effect
of NaCl stress on the growth of germinating soybean. In contrast, the above effects were reversed by combination with
EGTA. Under the supplemental CaCl2 treatment, there was no significant difference in GABA content in the cotyledon and
embryo of germinating soybean compared with NaCl stress treatment alone but it was significantly higher than the control.
Furthermore, when aminoguanidine (AG), an amine oxidase inhibitor, was added under NaCl plus CaCl2 treatment, GABA
content in the cotyledon and embryo was decreased by 17.1% and 26.5%, respectively. In addition, AG added under NaCl
combined with EGTA treatment resulted in a decrease in GABA content in cotyledon and embryo by 9.8% and 8.5%,
respectively. The present study indicates that exogenous calcium countered the harmful effect of salt stress and increased
the biomass and GABA content of germinating soybeans. Moreover, CaCl2 or EGTA addition decreased the contribution of
polyamine degradation pathway to GABA accumulation compared to NaCl stress.

Key words: soybean, germination, gamma-aminobutyric acid, NaCl stress

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