基于体外模拟消化的黑脉羊肚菌多酚细胞抗氧化及抗增殖活性

doi:10.7506/spkx1002-6630-201621040

食品科学

基于体外模拟消化的黑脉羊肚菌多酚细胞抗氧化及抗增殖活性

郑少杰1，廖霞1，卢可可1，刘冬2，吴素蕊3，明建1,*

1.西南大学食品科学学院，重庆 400715；2.深圳职业技术学院应用化学与生物技术学院，广东深圳 518055；
3.中华全国供销合作总社昆明食用菌研究所，云南昆明 650223

出版日期:2016-11-15 发布日期:2016-11-18

Antioxidant and Antiproliferative Activity in Human Cancer Cells of Polyphenols Extracted from Morchella angusticepes Peck Based on in Vitro Simulated Digestion

ZHENG Shaojie1, LIAO Xia1, LU Keke1, LIU Dong2, WU Surui3, MING Jian1,*

1. College of Food Science, Southwest University, Chongqing 400715, China;
2. School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, China;
3. Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooporatives, Kunming 650223, China

Online:2016-11-15 Published:2016-11-18

摘要/Abstract

摘要：

以黑脉羊肚菌多酚提取物为研究对象，通过体外模拟消化实验，研究消化过程中消化液多酚含量及氧自由基吸收能力的变化；研究体外模拟胃、肠消化对人肝癌细胞（HepG2细胞）和人结肠癌细胞（Caco-2细胞）的毒性及抗增殖活性的影响；并以HepG2细胞为模型对细胞抗氧化活性（cellular antioxidant activity，CAA）进行评价。结果表明：体外模拟消化后，黑脉羊肚菌多酚释放及氧自由基吸收能力均有提高，模拟胃、肠消化过程中多酚释放分别在1 h和0.5 h达到最高并保持稳定。细胞实验表明，在不产生细胞毒性的范围内，胃0 h、胃液1 h、肠液0.5 h对HepG2的抑制率分别为41%、45%、91%；对Caco-2细胞的抑制率分别为73%、96%、90%。CAA结果显示，胃消化处理的样品表现出促氧化的作用，肠消化处理的样品表现出抗氧化的作用，肠液0.5 h的CAA值为（70.506±0.011）μmol QE/100 g（以干质量计）。由此可见，体外模拟消化处理黑脉羊肚菌具有一定的抗氧化及抗增殖活性，为羊肚菌功能活性的开发提供了理论依据。

关键词: 黑脉羊肚菌, 模拟消化, 多酚, 氧自由基吸收能力, 细胞抗氧化活性

Abstract:

Morchella angusticepes Peck was subjected to in vitro simulated gastrointestinal digestion. Changes in
polyphenol content and antioxidant activity in terms of oxygen radical absorption capacity (ORAC) were determined during
the digestion process. The anti-proliferative activity and cytotoxicity of digests were measured in HepG2 cells and Caco-2
cells as a function of digestion time. Antioxidant activity was evaluated as well by cellular antioxidant activity (CAA) assay
in HepG2 cells. The results showed that after the gastrointestinal digestion, polyphenol content and ORAC were increased
significantly. Polyphenol release reached the maximum level and then remained stable after 1 h gastric digestion and 0.5 h
intestinal digestion, respectively. The percentage inhibition of HepG2 cells by Morchella angusticepes Peck and its 1 h
gastric and 0.5 h intestinal digests were 41%, 45% and 91%, respectively, and the percentage inhibition of Caco-2 cells
were 73%, 96% and 90%, respectively. The gastric digests showed pro-oxidant effect, whereas the intestinal digests showed
antioxidant capacity. The CAA value of the 0.5 h intestinal digest was (70.506 ± 0.011) μmol QE/100 g md. In conclusion,
in vitro digestion of Morchella angusticepes Peck showed anti-proliferation and cellular antioxidant activity, which could
provide a theoretical basis for further development and utilization of Morchella angusticepes Peck.

Key words: Morchella angusticepes Peck, simulated digestion, polyphenols, oxygen radical absorbance capacity, cellular antioxidant activity

中图分类号:

R151.2

郑少杰，廖霞，卢可可，刘冬，吴素蕊，明建. 基于体外模拟消化的黑脉羊肚菌多酚细胞抗氧化及抗增殖活性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201621040.

ZHENG Shaojie, LIAO Xia, LU Keke, LIU Dong, WU Surui, MING Jian. Antioxidant and Antiproliferative Activity in Human Cancer Cells of Polyphenols Extracted from Morchella angusticepes Peck Based on in Vitro Simulated Digestion[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201621040.

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基于体外模拟消化的黑脉羊肚菌多酚细胞抗氧化及抗增殖活性

Antioxidant and Antiproliferative Activity in Human Cancer Cells of Polyphenols Extracted from Morchella angusticepes Peck Based on in Vitro Simulated Digestion

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