MeJA处理对蓝莓果实采后灰霉病的影响及机理

doi:10.7506/spkx1002-6630-201622047

摘要/Abstract

摘要： 以“埃利奥特”蓝莓果实为实验材料，研究茉莉酸甲酯（methyl jasmonate，MeJA）处理对蓝莓果实灰霉病、活性氧代谢酶、防御相关酶活性和总酚、总花色苷含量的影响。50 μmol/L MeJA处理蓝莓果实后刺孔接种Botrytis cinerea孢子悬浮液，于（1±1） ℃贮藏16 d。结果表明，MeJA处理有效抑制果实发病率和过氧化氢酶、抗坏血酸过氧化物酶活性，促进贮藏初期果实H2O2的迅速积累，提高果实防御相关酶苯丙氨酸解氨酶、过氧化物酶和多酚氧化酶活性，促进酚类、木质素等植保素类物质的合成。表明MeJA处理可提高蓝莓果实抗病性，从而有效抑制了果实灰霉病的发生。

关键词: 茉莉酸甲酯, 活性氧代谢, 防御相关酶, 抗病性, 灰霉病

Abstract: The effects of methyl jasmonate (MeJA) treatment on postharvest grey mould decay, the activities of enzymes involved in reactive oxygen species (ROS) metabolism, defense-related enzymes activities and total phenol and total anthocyanin contents in ‘Elliott’ blueberry were investigated. Blueberry fruits were treated with MeJA at 50 μmol/L, inoculated with Botrytis cinerea and then stored at (1 ± 1) ℃ for 16 days. MeJA treatment resulted in significantly lower disease incidence compared with control fruits. MeJA treatment significantly inhibited the activities of catalase (CAT) and ascorbate peroxidase (APX) and thus resulted in a higher level of H2O2 in the early period of storage. However, MeJA treatment enhanced the activities of phenylalanine ammonialyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD), and induced the synthesis of phenolics and lignin. These results suggest that MeJA treatment can reduce the incidence and severity of grey mould decay by enhancing the disease resistance of blueberry fruit.

Key words: methyl jasmonate, reactive oxygen species metabolism, defense-related enzymes, disease resistance, gray mould decay

中图分类号:

TS255.3

黄晓杰,李婧,柴媛,韩媛媛,高嘉悦. MeJA处理对蓝莓果实采后灰霉病的影响及机理[J]. 食品科学, 2016, 37(22): 307-312.

HUANG Xiaojie, LI Jing, CHAI Yuan, HAN Yuanyuan, GAO Jiayue. Effect and Mechanism of Methyl Jasmonate on Incidence of Grey Mould Decay in Postharvest Blueberry[J]. FOOD SCIENCE, 2016, 37(22): 307-312.

参考文献

[1] PANIAGUA A, EAST A, HEYES J. Interaction of temperature control deficiencies and atmosphere conditions during blueberry storage on quality outcomes[J]. Postharvest Biology and Technology, 2014, 95: 50-59. DOI:10.1016/j.postharvbio.2014.04.006.
[2] YANG Guiyun, YUE Jin, GONG Xincheng, et al. Blueberry leaf extracts incorporated chitosan coatings for preserving postharvest quality of fresh blueberries[J]. Postharvest Biology and Technology, 2014, 92: 46-53. DOI:10.1016/j.postharvbio.2014.01.018.
[3] ?ZTüRK B, ALTUNTAS E, YILDIZ K, et al. Effect of methyl jasmonate treatments on the bioactive compounds and physicochemical quality of ‘Fuji’ apples[J]. Ciencia e Investigación Agraria, 2013, 40(1): 201-212. DOI:10.4067/S0718-16202013000100018.
[4] CONCHA C M, FIGUEROA N E, POBLETE L A, et al. Methyl jasmonate treatment induces changes in fruit ripening by modifying the expression of several ripening genes in Fragaria chiloensis fruit[J]. Plant Physiology and Biochemistry, 2013, 70: 433-444. DOI:10.1016/j.plaphy.2013.06.008.
[5] WASTERNACK C, PARTHIER B. Jasmonate-signalled plant gene expression[J]. Trends in Plant Science, 1997, 2(8): 302-307. DOI:10.1016/S1360-1385(97)89952-9.
[6] MOLINE H, BUTA J, SAFTNER R, et al. Comparison of three volatile natural products for the reduction of postharvest decay in strawberries[J]. Advances in Strawberry Research, 1997, 16: 43-48.
[7] YAO Hongjie, TIAN Shiping. Effects of pre- and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage[J]. Postharvest Biology and Technology, 2005, 35(3): 253-262. DOI:10.1016/j.postharvbio.2004.09.001.
[8] YAO Hongjie, TIAN Shiping. Effects of a biocontrol agent and methyl jasmonate on postharvest diseases of peach fruit and the possible mechanisms involved[J]. Journal of Applied Microbiology, 2005, 98(4): 941-950. DOI:10.1111/j.1365-2672.2004.02531.x.
[9] HUANG Xiaojie, LI Jing, SHANG Hongli, et al. Effect of methyl jasmonate on the anthocyanin content and antioxidant activity of blueberries during cold storage[J]. Journal of the Science of Food and Agriculture, 2015, 95(2): 337-343. DOI:10.1002/jsfa.6725.
[10] 曹士锋. 枇杷果实采后品质劣变调控及其机理研究[D]. 南京: 南京农业大学, 2008: 47.
[11] 曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007: 101-144.
[12] SLINKARD K, SINGLETON V L. Total phenol analysis: automation and comparison with manual methods[J]. American Journal of Enology and Viticulture, 1977, 28(1): 49-55.
[13] LAKO J, TRENERRY V C, WAHLQVIST M, et al. Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods[J]. Food Chemistry, 2007, 101(4): 1727-1741. DOI:10.1016/j.foodchem.2006. 01.031.
[14] MORRISON I. A semi-micro method for the determination of lignin and its use in predicting the digestibility of forage crops[J]. Journal of the Science of Food and Agriculture, 1972, 23(4): 455-463. DOI:10.1002/jsfa.2740230405.
[15] 田世平, 罗云波, 王贵禧. 园艺产品采后生物学基础[M]. 北京: 科学出版社, 2011: 43-55; 312-318.
[16] WANG Kaituo, JIN Peng, HAN Lin, et al. Methyl jasmonate induces resistance against Penicillium citrinum in Chinese bayberry by priming of defense responses[J]. Postharvest Biology and Technology, 2014, 98: 90-97. DOI:10.1016/j.postharvbio.2014.07.009.
[17] DING C K, WANG C, GROSS K C, et al. Jasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit[J]. Planta, 2002, 214(6): 895-901. DOI:10.1007/s00425-001-0698-9.
[18] JIN P, ZHENG Y, TANG S, et al. Enhancing disease resistance in peach fruit with methyl jasmonate[J]. Journal of the Science of Food and Agriculture, 2009, 89(5): 802-808. DOI:10.1002/jsfa.3516.
[19] ZHOU Y, LI S, ZENG K. Exogenous nitric oxide-induced postharvest disease resistance in citrus fruit to Colletotrichum gloeosporioides[J]. Journal of the Science of Food and Agriculture, 2016, 96(2): 505-512. DOI:10.1002/jsfa.7117.
[20] JIANG L, JIN P, WANG L, et al. Methyl jasmonate primes defense responses against Botrytis cinerea and reduces disease development in harvested table grapes[J]. Scientia Horticulturae, 2015, 192: 218-223 . DOI:10.1016/j.scienta.2015.06.015.
[21] CAO Shifeng, ZHENG Yonghua, YANG Zhenfeng, et al. Effect of methyl jasmonate on the inhibition of Colletotrichum acutatum infection in loquat fruit and the possible mechanisms[J]. Postharvest Biology and Technology, 2008, 49(2): 301-307. DOI:10.1016/j.postharvbio.2007.12.007.
[22] REN Yalin, WANG Yunfei, BI Yang, et al. Postharvest BTH treatment induced disease resistance and enhanced reactive oxygen species metabolism in muskmelon (Cucumis melo L.) fruit[J]. European Food Research and Technology, 2012, 234(6): 963-971. DOI:10.1007/s00217-012-1715-x.
[23] NEILL S, DESIKAN R, HANCOCK J. Hydrogen peroxide signalling[J]. Current Opinion in Plant Biology, 2002, 5(5): 388-395. DOI:10.1016/S1369-5266(02)00282-0.
[24] REIMERS P J, LEACH J E. Race-specific resistance to Xanthomonas oryzae pv. oryzae conferred by bacterial blight resistance gene Xa-10 in rice (Oryza sativa) involves accumulation of a lignin-like substance in host tissues[J]. Physiological and Molecular Plant Pathology, 1991, 38(1): 39-55. DOI:10.1016/S0885-5765(05)80141-9.
[25] CHITTOOR J M, LEACH J E, WHITE F F. Induction of peroxidase during defense against pathogens[C]//Pathogenesis-Related Proteins in Plants, CRC Press, 1999: 171-193.