仿刺参卵多糖的分离纯化及体外抗肿瘤活性

doi:10.7506/spkx1002-6630-201623018

食品科学

仿刺参卵多糖的分离纯化及体外抗肿瘤活性

刘昕1，刘京熙1，张健1,2,*，王婷3，王共明1，井月欣1，赵云苹1

1.山东省海洋资源与环境研究院，山东烟台 264006；2.山东省海洋生态修复重点实验室，山东烟台 264006；
3.上海海洋大学食品学院，上海 201306

出版日期:2016-12-15 发布日期:2016-12-21

Purification and Antitumor Activity in vitro of Polysaccharides from Apostichopus japonicus Spawn

LIU Xin1, LIU Jingxi1, ZHANG Jian1,2,*, WANG Ting3, WANG Gongming1, JING Yuexin1, ZHAO Yunping1

1. Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;
2. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China;
3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

Online:2016-12-15 Published:2016-12-21

摘要/Abstract

摘要： 目的：探究仿刺参卵多糖的结构及体外抗肿瘤活性。方法：通过酶解醇沉获得仿刺参卵粗多糖（polysaccharides from Apostichopus japonicus spawn，SPS）；通过色谱柱纯化后进行理化性质分析；采用噻唑蓝法研究卵多糖对HeLa、HGC-27细胞的体外增殖抑制作用。结果：获得3 种不同多糖组分SPS-1、SPS-2、SPS-3，对干预的肿瘤细胞均有显著抑制作用。SPS-1抑制作用最强，对HeLa及HGC-27细胞的最高抑制率分别为98.04%、99.4%。结论：从仿刺参卵中分离出3 种多糖组分，均表现出一定的抑制肿瘤细胞增殖活性。

关键词: 仿刺参卵, 多糖, 纯化, 抗肿瘤活性

Abstract: Purpose: To investigate the structural characteristics and antitumor activity in vitro of polysaccharides from
Apostichopus japonicus spawn. Methods: The polysaccharides from Apostichopus japonicus spawn (SPS) were isolated by
protease hydrolysis and alcohol precipitation, and then purified by chromatography. Their physicochemical properties were
analyzed. Their inhibitory effect on the growth of HeLa and HGC-27 cells was evaluated using methyl thiazolyl tetrazolium
assay. Results: SPS-1, SPS-2 and SPS-3 were purified from SPS. The proliferation of two tumor cells was inhibited by
intervention of each of the three polysaccharides. SPS-1 showed the strongest antitumor activity, with a percentage inhibition
of 98.04% and 99.4% for HeLa and HGC-27 cells, respectively. Conclusion: Three different polysaccharides with the ability
to inhibit tumor cell proliferation have been obtained from A. japonicus spawn.

Key words: Apostichopus japonicus spawn, polysaccharide, purification, antitumor activity

中图分类号:

TS254.1

刘昕，刘京熙，张健，王婷，王共明，井月欣，赵云苹. 仿刺参卵多糖的分离纯化及体外抗肿瘤活性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201623018.

LIU Xin, LIU Jingxi, ZHANG Jian, WANG Ting, WANG Gongming, JING Yuexin, ZHAO Yunping. Purification and Antitumor Activity in vitro of Polysaccharides from Apostichopus japonicus Spawn[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201623018.

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仿刺参卵多糖的分离纯化及体外抗肿瘤活性

Purification and Antitumor Activity in vitro of Polysaccharides from Apostichopus japonicus Spawn

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