食品科学 ›› 2017, Vol. 38 ›› Issue (2): 82-86.doi: 10.7506/spkx1002-6630-201702013

• 生物工程 • 上一篇    下一篇

巴氏醋杆菌TCA循环代谢对醋酸发酵的影响

殷海松,张仁宽,常燕钢,郑 宇,王 敏   

  1. 1.工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津 300457; 2.天津现代职业技术学院生物工程学院,天津 300350
  • 出版日期:2017-01-25 发布日期:2017-01-16
  • 基金资助:
    国家高技术研究发展计划(863计划)项目(2013AA102106);“十三五”国家重点研发计划重点专项(2016YFD0400505);教育部“长江学者和创新团队发展计划”项目(RT15R49);国家自然科学基金面上项目(31671851;31471722);天津市科技支撑计划项目(16YFZCNC00650);天津市应用基础及前沿技术研究计划项目(13JCQNJC10000)

Effects of TCA Cycle Metabolism on the Acetic Acid Fermentation of Acetobacter pasteurianus

YIN Haisong, ZHANG Renkuan, CHANG Yangang, ZHENG Yu, WANG Min   

  1. 1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; 2. School of Bioengineering, Tianjin Modern Vocational Technology College, Tianjin 300350, China
  • Online:2017-01-25 Published:2017-01-16

摘要: 巴氏醋杆菌是醋酸发酵常用微生物,通过添加醋酸、三羧酸(tricarboxylic acid,TCA)循环抑制剂和中间产物干扰TCA循环能量代谢,研究其对菌体生长和醋酸发酵的影响。研究结果发现,添加质量分数1%的醋酸,胞内TCA代谢关键酶的表达量出现上调,胞内ATP含量增加了125%,表明能够强化TCA循环能量代谢,促进菌体生长。添加TCA循环抑制剂抑制TCA循环能量代谢,巴氏醋杆菌菌体生长和产酸受到显著抑制,菌体生物量分别降低了90%和87%,产酸量分别降低了90%和94%。添加0.05%草酰乙酸、琥珀酸、苹果酸等TCA中间产物,巴氏醋杆菌胞内ATP含量分别提高了202%、185%和165%,表明添加草酰乙酸等中间物质能够显著提高TCA循环偶联呼吸链产能,发酵72 h时,菌体生物量分别提高了92%、106%和104%,菌体产酸量分别提高了30%、33%和31%。实验结果表明,TCA循环能量代谢对巴氏醋杆菌菌体生长和产酸产生显著影响,TCA能量代谢的强化对醋酸发酵产生正向作用。

 

关键词: 巴氏醋酸杆菌, TCA循环, 能量代谢, 醋酸, ATP含量

Abstract: The most commonly used microorganism for acetic acid fermentation is Acetobacter pasteurianus. In this investigaton, tricarboxylic acid cycle (TCA cycle) energy metabolism in Acetobacter pasteurianus was interfered by adding acetic acid, TCA cycle inhibitors, and intermediate metabolites. The effects of energy metabolism on the cell growth and acetic acid fermentation of Acetobacter pasteurianus were studied. Results showed that addition of 1% acetic acid obviously strengthened intracellular TCA cycle energy metabolism. The expression levels of the key enzymes involved in the intracellular TCA cycle were significantly up-regulated, and intracellular ATP concentration was increased by 125% compared with that without adding acetic acid. TCA cycle energy metabolism was inhibited by adding TCA cycle inhibitors, the cell growth and acetic acid concentration were significantly inhibited and bacterial biomass was reduced by 90% and 87%, respectively. Acetic acid concentration was reduced by 90% and 94%, respectively. Addition of 0.05% intermediates metabolites (oxaloacetic acid, malic acid, and succinic acid) increased intracellular ATP concentration by 202%, 185% and 165%, respectively, suggesting significantly enhanced TCA cycle-coupled respiratory chain energy metabolism. Bacterial biomass was increased by 92%, 106% and 104% respectively, and acetic acid concentration was increased by 30%, 33% and 31%, respectively, after fermentation for 72 h. Our findings show that TCA cycle energy metabolism has a significant impact on the bacterial growth and acetic acid production of Acetobacter pasteurianus and that strengthening TCA energy metabolism has a positive effect on acetic acid fermentation.

Key words: Acetobacter pasteurianus, tricarboxylic acid cycle, energy metabolism, acetic acid, ATP concentration

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