食品科学 ›› 2017, Vol. 38 ›› Issue (2): 255-263.doi: 10.7506/spkx1002-6630-201702040

• 工艺技术 • 上一篇    下一篇

淫羊藿叶多糖工艺优化及抗氧化活性

谭 莉,陈瑞战,常清泉,陆 娟,金辰光,殷 薇   

  1. 长春师范大学化学学院,吉林 长春 130032
  • 出版日期:2017-01-25 发布日期:2017-01-16
  • 基金资助:
    吉林省教育厅“十二五”科学技术研究项目([2014]258;[2015]355)

Optimization of Extraction and Antioxidant Activity of Polysaccharides from Epimedium Leaves

TAN Li, CHEN Ruizhan, CHANG Qingquan, LU Juan, JIN Chenguang, YIN Wei   

  1. College of Chemistry, Changchun Normal University, Changchun 130032, China
  • Online:2017-01-25 Published:2017-01-16

摘要: 探索和优化超声复合酶解提取淫羊藿叶粗多糖工艺。在单因素试验的基础上,通过正交试验优化复合酶添加量,再采用Box-Behnken设计和响应面优化超声复合酶解提取工艺参数。结果显示:不同酶添加量对多糖提取得率的影响次序是:纤维素酶>果胶酶>木瓜蛋白酶>α-淀粉酶,最佳复合酶(木瓜蛋白酶、果胶酶、纤维素酶和α-淀粉酶)的添加量分别为50、250、200、100 U/g;最佳提取条件为提取温度46.8 ℃、超声时间42.3 min、pH 4.3、超声功率311 W。在此实验条件下,粗多糖提取得率为5.98%,与模型预测值(6.2%)接近。粗多糖通过琼脂糖离子交换和葡聚糖分子筛凝胶柱色谱分离纯化,得到3 个主要多糖组分(EPs-1、EPs-2、EPs-3),多糖组分采用DPPH自由基、羟自由基、超氧阴离子自由基清除和铁离子还原能力实验进行了抗氧化活性评价。结果表明,超声复合酶提取作为一个高效和环保的提取技术,可以应用于从植物原料中提取活性成分;抗氧化活性实验显示3 个多糖组分都具有显著的抗氧化活性,其活性呈添加量依赖关系。这些结果说明淫羊藿多糖可以探索作为潜在的抗氧剂应用于功能食品和药品。

关键词: 淫羊藿多糖, 超声复合酶, 响应面, 抗氧化活性

Abstract: In this study, an ultrasonic-assisted enzymatic extraction (UAEE) method was proposed and optimized for the extraction of polysaccharides (CEPs) from Epimedium leaves. In the first step, the optimization of enzyme mixtures for the hydrolysis of Epimedium leaves was carried out by the combined use of one-factor-at-a-time method and orthogonal array design. Subsequently, the optimization of extraction parameters was done using Box-Behnken design with response surface methodology. The results showed that the influence of the dosage of enzymes on the extraction yield of CEPs was in the following order: cellulose > pectinase > papain > α-amylase, and the optimal combination found were papain 50 U/g, pectinase 250 U/g, cellulase 200 U/g and α-amylase 100 U/g. The optimal extraction parameters were determined as 46.8 ℃, 42.3 min, 4.3 and 311 W for temperature, time, pH, and ultrasonic power, respectively. Under these conditions, the experimental yield of CEPs was 5.98%, which was well in close agreement with the value (6.2%) predicted by the proposed model. Three major fractions (EPs-1, EPs-2 and EPs-3) from the CEPs were purified by DEAE-Sepharose fast-flow and Sephadex G-100 column chromatography. The antioxidant activities of the three fractions were evaluated by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide anion racial scavenging capacity assays, and ferricreducing antioxidant power (FRAP) assay in vitro. It was indicated that UCEE could be an effective and environmentfriendly technique for extracting active ingredients from plant materials. All the three polysaccharides exhibited significant antioxidant activities in a dose-dependent manner. These results suggested that Epimedium polysaccharides could be explored as potential antioxidants for use in functional foods or medicines.

Key words: Epimedium polysaccharide, ultrasonic-assisted enzymatic extraction (UAEE), response surface methodology, antioxidant activity

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