食品科学 ›› 2012, Vol. 33 ›› Issue (3): 131-135.doi: 10.7506/spkx1002-6630-201703028

• 生物工程 • 上一篇    下一篇

镰刀菌(Fusarium sp. B158)色素的提取及其理化性质

曾祥龙,蔡宇杰,廖祥儒,曾祥康,李枝玲,张大兵   

  1. 1.江南大学 工业生物技术教育部重点实验室 2.江苏汉邦科技有限公司
  • 发布日期:2012-02-14
  • 基金资助:
    中央高校基本科研业务费专项资金资助项目(JUSRP21120);国家自然科学基金项目(21045007); 江苏省科技创新与成果转化(重大科技支撑与自主创新)专项引导资金项目(BY2010117)

Extraction and Physico-chemical Properties of Pigments from Liquid-Cultured Mycelia of Fusarium sp. B158

ZENG Xiang-long,CAI Yu-jie,LIAO Xiang-ru,ZENG Xiang-kang,LI Zhi-ling,ZHANG Da-bing   

  1. 1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;2. Jiangsu Hanbon Science and Technology Co. Ltd., Huai’an 223001, China
  • Published:2012-02-14

摘要: 从一株新型镰刀菌(Fusarium sp. B158)液态发酵菌丝体提取色素,并对其理化性质进行研究。结果表明:该色素的最佳提取溶剂是酸性乙醇(pH2)。色素的全波长扫描显示,该色素在324nm和360nm波长处均有吸收峰,实验,选择360nm作为色素的最大检测波长。色素的稳定性研究表明,色素对热、光、甜味剂以及常见食品添加剂表现稳定,对溶剂的pH值以及氧化剂和还原剂敏感。大部分的金属离子(Cu2+和Fe3+除外)对色素的稳定性影响不大。

关键词: 色素, 提取, 稳定性, 镰刀菌

Abstract: In this work, pigments from the liquid-cultured mycelia of Fusarium sp. B158 were extracted and their physico-chemical properties were investigated. The best solvent for extracting pigments from the liquid-cultured mycelia of Fusarium sp. B158 was found to be acidified ethanol (pH = 2). The extract obtained showed maximum absorption at 324 nm and 360 nm. To facilitate further investigations, 360 nm was chosen as maximum absorption wavelength. The pigments were stable to heat, light, sugars and common food additives, but sensitive to pH, oxidizers and reductants. Most of the investigated metal ions (except Cu2+ and Fe3+) revealed little effect on the pigments.

Key words: pigment, extraction, stability, Fusarium sp. B158

中图分类号: