食品科学 ›› 2012, Vol. 33 ›› Issue (3): 203-206.doi: 10.7506/spkx1002-6630-201703042

• 生物工程 • 上一篇    下一篇

DiversiLab系统沙门氏菌分子分型评价

许龙岩,袁慕云,林文丽,张旺,焦 红   

  1. 广东出入境检验检疫局技术中心食品实验室
  • 发布日期:2012-02-14
  • 基金资助:
    广东省科技计划项目(2009B0600013);广东出入境检验检疫局科研项目(2009GDK35)

Molecular Typing Evaluation of Salmonella in Diversilab System

XU Long-yan,YUAN Mu-yun,LIN Wen-li,ZHANG Wang,JIAO Hong   

  1. Food Laboratory of Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China
  • Published:2012-02-14

摘要: 目的:评价DiversiLab(DVL)系统对沙门氏菌(SA)的分型能力。方法:用rep-PCR技术为原理的DVL系统对进出口食品中分离的44株SA进行分子分型,并与脉冲场凝胶电泳(PFGE)结果比较,探讨DVL对SA的种群分类能力和分辨率。结果:44株SA经rep-PCR分型分成22个群,分离自不同国家、不同食品中的相同血清型SA分布在同一个群,而且菌株之间相似性非常高。在rep-PCR结果中分在同一个群中的SA,在PFGE结果中除SA2和SA15外,其他菌株之间相关性较低。结论:DVL在SA的种群的分类能力上优于PFGE,但分辨率低于PFGE。

关键词: 沙门氏菌, DiversiLab系统, rep-PCR, PFGE, 分子分型

Abstract: Objective: To evaluate the typing performance of DiversiLab system (DVL) for Salmonella. Method: A DVL system, which is based on rep-PCR, was used to type 44 Salmonella isolates from exported and imported foods and compared with pulsed-field gel electrophoresis (PFGE). The Salmonella isolates were typed by rep-PCR into 22 groups. The same serotypes of Salmonella isolates from different countries and foods were distributed in the same group with a high similarity. The PFGE results obtained for Salmonella isolates assigned to the same group using rep-PCR indicated a low similarity among all other isolates except No. 2 and No. 15. Conclusion: DVL shows better typing ability but lower resolution for Salmonella than PFGE.

Key words: Salmonella, DiversiLab system, rep-PCR, PFGE, molecular typing

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