食品科学 ›› 2017, Vol. 38 ›› Issue (4): 118-124.doi: 10.7506/spkx1002-6630-201704020

• 生物工程 • 上一篇    下一篇

α-淀粉酶抑制剂生物合成途径和代谢流分析

张洪志,徐庆阳,曹华杰,白 芳,陈 宁,白 钢   

  1. 1.天津科技大学生物工程学院,代谢控制发酵技术国家地方联合工程实验室,天津 300457; 2.南开大学药学院,天津 300071
  • 出版日期:2017-02-25 发布日期:2017-02-28
  • 基金资助:
    天津市应用基础与前沿技术研究计划项目(14JCYBJC28500)

Application of Biosynthesis Pathway Analysis and Metabolic Flux Analysis for Optimization of Fermentation of α-Amylase Inhibitor

ZHANG Hongzhi, XU Qingyang, CAO Huajie, BAI Fang, CHEN Ning, BAI Gang,   

  1. 1. China National and Local United Engineering Lab of Metabolic Control Fermentation Technology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; 2. College of Pharmacy, Nankai University, Tianjin 300071, China
  • Online:2017-02-25 Published:2017-02-28

摘要: 为提高糖类的利用效率,加强糖类代谢向生成α-淀粉酶抑制剂的方向流动,提高α-淀粉酶抑制剂产量,对天蓝黄链霉菌合成α-淀粉酶抑制剂的代谢网络进行分析,找出影响α-淀粉酶抑制剂合成的代谢流量分配规律和关键节点,并且应用代谢流分析的方法研究了谷氨酸钠对α-淀粉酶抑制剂发酵中后期胞内代谢流分布的影响。结果表明:在α-淀粉酶抑制剂分批发酵过程中,未流加谷氨酸钠时合成α-淀粉酶抑制剂的代谢流量为1.84;在发酵培养基中流加谷氨酸钠使其质量浓度维持在4.0 g/L后,α-淀粉酶抑制剂生物合成的代谢流增长至3.18。因此发酵过程中流加谷氨酸钠能够改变α-淀粉酶抑制剂生物合成途径的关键节点6-磷酸葡萄糖、7-磷酸景天庚酮糖及谷氨酸的代谢流分布,提高α-淀粉酶抑制剂生物合成途径的代谢流量。

关键词: α-淀粉酶抑制剂, 代谢流分析, 谷氨酸钠

Abstract: This study aimed to improve the yield of α-amylase inhibitor and the efficiency of carbohydrate utilization during the fermentation of Streptomyces coelicoflavus (S. coelicoflavus). The metabolic network leading to the biosynthesis of α-amylase inhibitor was analyzed to find out the key nodes which influenced the production of α-amylase inhibitor. The effects of sodium glutamate on the metabolic flux distributions during the middle and late periods of fermentation were studied, and metabolic flux analysis for α-amylase inhibitor production at pseudo-steady state was also conducted. The results showed that the metabolic flux channeled to the α-amylase inhibitor biosynthesis pathway was 1.84 in batchwise fermentation without the addition of sodium glutamate, while, with the addition of sodium glutamate (the concentration was maintain at 4.0 g/L), the metabolic flux was 3.18, suggesting that the addition of sodium glutamate could change the metabolic flux distributions of the key nodes (glucose-6-phosphate, sedoheptulose-7-phosphate, and glutamate) and enhance the biosynthesis of α-amylase inhibitor.

Key words: α-amylase inhibitor, metabolic flux analysis, sodium glutamate

中图分类号: