食品科学 ›› 2017, Vol. 38 ›› Issue (4): 275-282.doi: 10.7506/spkx1002-6630-201704045

• 安全检测 • 上一篇    下一篇

氢核磁共振结合正交偏最小二乘法对油菜蜜中果葡糖浆掺假的判别分析

陈 雷,刘红兵,罗立廷   

  1. 中国科学院武汉物理与数学研究所,波谱与原子分子物理国家重点实验室,武汉磁共振中心,湖北 武汉 430071
  • 出版日期:2017-02-25 发布日期:2017-02-28
  • 基金资助:
    中国科学院仪器设备功能开发技术创新项目(YG2011095)

Detection of Honey Adulteration with High Fructose Syrups Using 1H Nuclear Magnetic Resonance and Orthogonal Partial Least Squares

CHEN Lei, LIU Hongbing, LUO Liting   

  1. National Center for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China
  • Online:2017-02-25 Published:2017-02-28

摘要: 利用氢核磁共振(1H nuclear magnetic resonance,1H NMR)谱图结合正交偏最小二乘(orthogonal partialleast squares,OPLS)法对油菜蜂蜜和果葡糖浆掺假蜂蜜进行判别分析。采集了303 个油菜蜜样品和180 个按照不同比例配制的果葡糖浆掺假蜂蜜样品的1H NMR谱图,并对油菜蜜主要糖类成分和部分低含量化合物进行了信号归属。采用OPLS对训练集数据进行分析,建立蜂蜜果葡糖浆掺假判别模型。通过排列实验法对模型进行可靠性检验。结果显示,油菜蜜和果葡糖浆掺假蜂蜜样品在OPLS得分图中能明显区分。训练集和测试集样品的总体判别正确率分别为98.40%和98.24%。因此,1H NMR与OPLS相结合可以实现油菜蜜中果葡糖浆掺假的快速鉴别。该方法是基于对蜂蜜成分的整体分析,避免了仅仅分析个别成分指标的检验方法中存在的缺陷,为蜂蜜质量监控提供了一种新思路。

关键词: 蜂蜜, 核磁共振, 正交偏最小二乘法, 掺假

Abstract: Combination of 1H nuclear magnetic resonance (1H NMR) spectroscopy and orthogonal partial least squares (OPLS) was successfully employed to detect rape honey adulteration with high fructose syrups. Three hundred and three authentic and 180 adulterated honey samples were analyzed. Glucose, sucrose and 13 minor components in honey samples were detected and identified from their 1H NMR spectra. The OPLS model based on NMR data was applied to detect adulteration in honey falsified by intentional addition of different concentrations of high fructose syrups. A distinct discrimination between authentic and adulterated honey samples was achieved in OPLS score plot. Overall classification rates of training set and testing set were 98.40% and 98.24%, respectively. Hence, 1H NMR spectroscopy coupled with OPLS offered a rapid and accurate tool for honey adulteration detection. The method avoided the disadvantage of monocomponent analysis and provided a potential standard for quality control of honey.

Key words: honey, nuclear magnetic resonance (NMR), orthogonal partial least squares (OPLS), adulteration

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