食品科学

• 基础研究 • 上一篇    下一篇

不同类型蛋白质水溶液的介电特性

孔繁荣,郭文川*,李伟强,王东阳   

  1. 西北农林科技大学机械与电子工程学院,陕西 杨凌 712100
  • 出版日期:2017-03-15 发布日期:2017-03-28

Dielectric Properties of Different Types of Protein in Aqueous Solutions

KONG Fanrong, GUO Wenchuan*, LI Weiqiang, WANG Dongyang   

  1. College of Mechanical and Electronic Engineering, Northwest A&F University, Yangling 712100, China
  • Online:2017-03-15 Published:2017-03-28

摘要: 为了解不同类型蛋白质水溶液的介电特性,为液态食品的蛋白质掺假提供新的检测方法,本实验采用同轴探头技术,研究了室温25 ℃、20~4 500 MHz频率范围内不同质量分数(0%~4.63%)的蛋白质(大豆蛋白、乳清蛋白和卵蛋白)水溶液的相对介电常数ε’和介质损耗因数ε”的变化规律。结果表明,随着频率的增大ε’逐渐减小,而ε”先减小后增大;当蛋白质质量分数相同时,大豆和乳清蛋白水溶液的ε’和ε”值均比较接近,与卵蛋白水溶液之间存在明显差异;在特定的频率段内,蛋白质水溶液的ε’(160~600 MHz和1 400~4 500 MHz)和ε”(20~1 400 MHz)与蛋白质质量分数间存在较好的线性相关性,其相关系数分别达到0.90和0.99。研究表明,不同类型和质量分数的蛋白质水溶液介电特性的变化规律不同,这使得基于介电特性检测蛋白饮料中蛋白质的类型及其质量分数成为可能。

关键词: 大豆蛋白, 乳清蛋白, 卵蛋白, 质量分数, 介电特性

Abstract: To understand the dielectric properties of different types of protein in aqueous solutions and further to develop a method for detecting protein adulteration of liquid foods, an open-ended coaxial-line probe was used to explore the changing patterns of the dielectric constant ε’ and dielectric loss factor ε” of aqueous protein solutions (soy protein, whey protein and egg albumin) at a concentration of 0%–4.63% in the frequency range of 20–4 500 MHz at 25 ℃. The results showed that ε’ decreased with increasing frequency, and ε” decreased at first and then increased. At the same protein concentration, the values of ε’ and ε” in soy protein and whey protein solutions had no significant difference, but they were smaller than those in egg albumin at a certain frequency. There were good linear correlations between protein concentration and dielectric properties with a correlation coeffiient of 0.90 and 0.99, respectively. This study indicated that different proteins and their concentration had different influences on dielectric properties of protein solutions, which makes it possible to detect protein types and concentration in protein-based beverage based on dielectric properties.

Key words: soy protein, whey protein, egg albumin, mass fraction, dielectric properties

中图分类号: