食品科学

• 营养卫生 • 上一篇    下一篇

秀珍菇多糖对D-半乳糖致衰老小鼠的保护作用

孙玉军1,2,江昌俊3,*,任四海4   

  1. 1.安徽科技学院生命科学学院,安徽 凤阳 233100;2.安徽农业大学生命科学学院,安徽 合肥 230036;3.安徽农业大学茶与食品科技学院,茶树生物学与资源利用国家重点实验室,安徽 合肥 230036;4.安徽省农业科学院烟草研究所,安徽 合肥 230031
  • 出版日期:2017-03-15 发布日期:2017-03-28

Protective Effect of Polysaccharides from the Fruiting Body of Pleurotus geesteranus against D-Galactose-Induced Aging Mice

SUN Yujun1,2, JIANG Changjun3,*, REN Sihai4   

  1. 1. School of Life Science, Anhui Science and Technology University, Fengyang 233100, China; 2. College of Life Sciences, Anhui Agricultural University, Hefei 230036, China; 3. State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science and Technology, Anhui Agricultural University, Hefei 230036, China; 4. Tobacco Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Online:2017-03-15 Published:2017-03-28

摘要: 研究秀珍菇子实体多糖(polysaccharide from the fruiting body of Pleurotus geesteranus,PFP)对D-半乳糖致衰老小鼠的保护作用。通过腹腔注射60 mg/(kg·d)D-半乳糖建立衰老小鼠模型,同时灌胃50、100、200 mg/(kg·d)不同剂量的PFP,42 d后,采用试剂盒测定小鼠肝、脑组织中过氧化氢酶(catalase,CAT)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的活力和丙二醛(malondialdehyde,MDA)的含量;同时,对小鼠血清谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine aminotransferase,ALT)、碱性磷酸酶(alkaline phosphatas,ALP)活力以及脾脏指数、胸腺指数进行测定;苏木精-伊红染色观察肝、脑组织。结果表明,PFP能拮抗D-半乳糖引起的小鼠衰老,使小鼠肝、脑组织中的CAT、SOD、GSH-Px活力升高,MDA含量降低。与模型组相比,中、高剂量组PFP显著降低小鼠血清的AST、ALP、ALT活力(P<0.05,P<0.01),极显著提高脾脏指数和胸腺指数(P<0.01);组织切片显示PFP对小鼠肝、脑损伤具有保护作用。因此PFP具有良好的抗衰老作用,其机制可能与其提高机体的抗氧化能力、增强机体的免疫功能有关。

关键词: 秀珍菇, 多糖, D-半乳糖, 抗氧化, 抗衰老

Abstract: The anti-aging effect of polysaccharides from the fruiting body of Pleurotus geesteranus (PFP) on a D-galactoseinduced aging mouse model. The aged mouse model was established by the injection of D-galactose. These mice were simultaneously administrated by gavage with PFP at different dosages for 42 days. At 12 h after the last administration, the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the contents of malondialdehyde (MDA) in serum, liver and brain were measured by using commercial reagent kits. The activities of serum aspartate aminotransferase (AST), alkaline phosphatas (ALP) and alanine aminotransferase (ALT), and spleen and thymus indexes were also determined. The liver and brain were taken for histological analysis through HE staining. The results showed that PFP had antagonism effects on D-galactose-induced aging in mice, and could increase the activities of CAT, SOD and GSH-Px and reduce MDA levels in serum, and liver and brain tissues. Compared with the aging model group, the activities of serum AST, ALT, ALP in the medium- and high-dosage PFP group significantly decreased (P < 0.05, P < 0.01) in parallel with a highly significant increase in both spleen index and thymus index (P < 0.01). Histological sections showed that PFP displayed a significant protective effect on liver and brain injury induced by D-galactose. In conclusion, PFP has significant antioxidant and anti-aging effects. The underlying mechanism might be related to improving antioxidant function and enhancing immune function in the body.

Key words: Pleurotus geesteranus, polysaccharide, D-galactose, antioxidant, anti-aging

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