食品科学 ›› 2017, Vol. 38 ›› Issue (6): 13-19.doi: 10.7506/spkx1002-6630-201706003

• 生物工程 • 上一篇    下一篇

香蕉MaWRKY31转录因子的特性及对MaACS1、MaACO1和MaSAG1的调控

范中奇,卓远芳,符亚莉,谭小丽,邝健飞,陆旺金,陈建业   

  1. 华南农业大学园艺学院,亚热带农业生物资源保护与利用国家重点实验室,南方园艺产品保鲜教育部工程研究中心,广东省果蔬保鲜重点实验室,广东 广州 510642
  • 出版日期:2017-03-25 发布日期:2017-03-28
  • 基金资助:
    国家自然科学基金面上项目(31372111);国家香蕉现代农业产业技术体系项目(CARS-32-09)

Molecular Characterization of a Transcriptional Factor, MaWRKY31, and Its Regulation on MaACS1, MaACO1 and MaSAG1

FAN Zhongqi, ZHUO Yuanfang, FU Yali, TAN Xiaoli, KUANG Jianfei, LU Wangjin, CHEN Jianye   

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, China
  • Online:2017-03-25 Published:2017-03-28

摘要: 从香蕉果实中克隆得到1 个WRKY转录因子,命名为MaWRKY31。氨基酸序列比对及进化树分析发现MaWRKY31具有一个典型的WRKY结构域,与拟南芥WRKY31和番茄SlWRKY6同源性较高,且同属于第Ⅱ类b亚族。实时荧光定量聚合酶链式反应表明外源乙烯处理诱导了MaWRKY31的表达,并且随着香蕉果实的成熟衰老,其表达明显增强。亚细胞定位和转录活性分析显示MaWRKY31定位于细胞核,是一种核蛋白,并且具有转录激活活性。双荧光素酶瞬时表达分析显示,MaWRKY31可以激活乙烯合成基因MaACS1和MaACO1,以及成熟衰老相关基因MaSAG1的启动子活性。这些结果表明MaWRKY31可能通过调控乙烯合成和成熟衰老相关基因的表达来参与香蕉果实成熟衰老过程,加深了对香蕉果实成熟衰老转录调控机制的认识。

关键词: 香蕉, 果实成熟, WRKY转录因子, 转录调控

Abstract: WRKY proteins are a class of plant-specific transcriptional factors (TFs), which play important roles in plant growth and development, leaf senescence and hormonal signaling. However, their relationship with banana fruit ripening and senescence remains unknown. In the present work, a WRKY TF, termed MaWRKY31, was obtained from banana fruit. Analysis of deduced amino acid sequence and phylogenetic tree revealed that MaWRKY31 contained a typical WRKY domain showing high homology with Arabidopsis thaliana WRKY31, and belonged to the Ⅱb sub-group. Realtime quantitative polymerase chain reaction displayed that MaWRKY31 was induced by ethylene, and up-regulated following banana fruit ripening and senescence. Sub-cellular localization and transcriptional activity analysis revealed that MaWRKY31 was a nuclear protein with transcriptional activation activity. In addition, dual-luciferase transient expression assay demonstrated that MaWRKY31 could activate the promoter activities of the ethylene biosynthetic genes MaACS1 and MaACO1 and the ripening-related gene MaSAG1. Taken together, these results suggest that MaWRKY31 may be involved in banana fruit ripening and senescence, possibly via the regulation of the expression of genes related to ethylene biosynthesis and ripening, which deepens the understanding of the transcriptional regulation mechanism of banana fruit ripening and senescence.

Key words: banana, fruit ripening and senescence, WRKY transcription factors, transcriptional regulation

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