食品科学 ›› 2017, Vol. 38 ›› Issue (6): 74-80.doi: 10.7506/spkx1002-6630-201706012

• 生物工程 • 上一篇    下一篇

氯化锂诱变蛹虫草菌株液体发酵富集微量元素锌

王 陶,储渊明,陈宏伟,李 文,高明侠,董玉玮,李同祥,张传丽   

  1. 徐州工程学院 江苏省食品资源开发与质量安全重点建设实验室,江苏 徐州 221111
  • 出版日期:2017-03-25 发布日期:2017-03-28
  • 基金资助:
    国家自然科学基金面上项目(81273004;31270577);江苏省青蓝工程优秀骨干教师培养对象人才项目; 江苏省产学研联合创新基金项目(BY2014031);江苏省高校自然科学研究面上项目(15KJD550002); 江苏省食品资源开发与质量安全重点建设实验室开放课题(SPKF201412); 徐州工程学院校级重点培育项目(XKY2015104);徐州工程学院校级培育项目(XKY2014214)

Zinc Enrichment of Cordyceps militaris Cultured in Liquid Medium: Optimization of Medium Components and Culture Conditions and Strain Improvement by LiCl Mutagenesis

WANG Tao, CHU Yuanming, CHEN Hongwei, LI Wen, GAO Mingxia, DONG Yuwei, LI Tongxiang, ZHANG Chuanli   

  1. Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, Xuzhou 221111, China
  • Online:2017-03-25 Published:2017-03-28

摘要: 为更好地开发蛹虫草资源,以蛹虫草为富锌载体,进行发酵富锌培养条件优化,选择最佳质量分数的LiCl诱变蛹虫草,通过火焰原子吸收法检测诱变前后菌丝体中锌元素的含量。结果表明:蛹虫草富锌的最优碳源为蔗糖,质量分数为3%,最优氮源为蛋白胨,质量分数为3%,最佳培养时间为6 d,最佳接种量为5%,最佳装液量为100 mL/250 mL,培养基中添加的最佳ZnSO4质量浓度为100 μg/mL,此时,富锌率为18.54%。LiCl的最佳诱变条件为:LiCl质量分数0.15%。选育出富锌较高菌株5 株,最高富锌率达24.68%,比出发菌株提高了近42%,具有广阔的应用前景。

关键词: 蛹虫草, 微量元素, 锌, 富集, LiCl诱变

Abstract: In order to make better use of Cordyceps militaris, the optimization of medium components and culture conditions was investigated for improved zinc enrichment of C. militaris in liquid-sate fermentation. Meanwhile, the optimal LiCl concentration was chosen to mutate C. militaris. Finally, zinc concentrations in the mycelia of the parental and mutant strains were determined by graphite furnace atomic absorption spectrometry. Results showed that the optimal culture conditions for C. militaris to enrich zinc were as follows: 3% sucrose as the best carbon source, 3% peptone as the best nitrogen source, fermentation time, 6 d; inoculum size, 5%; medium volume, 100 mL/250 mL; and initial ZnSO4 concentration, 100 μg/mL. Under these conditions, the rate of zinc enrichment was 18.54%. The LiCl concentration of 0.15% was optimal for the mutation of C. militaris. Five strains with higher zinc enrichment ability were obtained, giving the highest rate of zinc enrichment of 24.68%, which was increased by nearly 42% as compared with that of the original strain. Therefore the mutants would have promising applications for industrial production.

Key words: Cordyceps militaris, microelement, zinc, enrichment, LiCl mutagenesis

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