食品科学 ›› 2017, Vol. 38 ›› Issue (6): 191-196.doi: 10.7506/spkx1002-6630-201706030

• 成分分析 • 上一篇    下一篇

超声萃取高效液相色谱法测定诺丽果粉中的莨菪亭、芦丁和槲皮素含量

沈宋利,杨 健,沈燕飞,唐泽辉,陈梅兰   

  1. 1.浙江树人大学生物与环境工程学院,浙江 杭州 310015;2.美国关岛大学自然与应用科学学院,西太平洋热带研究中心,美国 关岛 GU 96923
  • 出版日期:2017-03-25 发布日期:2017-03-28
  • 基金资助:
    浙江省自然科学基金项目(Z14B070001)

Determination of Scopoletin, Rutin, and Quercetin in Noni (Morinda citrifolia) Fruit Powder by Ultrasonic Extraction Coupled with High Performance Liquid Chromatography

SHEN Songli, YANG Jian, SHEN Yanfei, TANG Zehui, CHEN Meilan,   

  1. 1. College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China; 2. Western Pacific Tropical Research Center, College of Natural and Applied Sciences, University of Guam, Mangilao, Guam GU 96923, USA
  • Online:2017-03-25 Published:2017-03-28

摘要: 采用超声萃取高效液相色谱法测定诺丽果粉中的莨菪亭、芦丁和槲皮素含量。为了提高萃取效率,实验系统地优化了影响萃取效率的因素(有机溶剂种类、有机溶剂与水的比例、萃取时间、萃取温度和超声频率)。结果表明,最佳萃取条件:萃取剂为体积分数80%甲醇溶液,萃取时间为30 min,萃取温度为20 ℃,超声频率为45 kHz。采用依利特Hypersil ODS柱(4.6 mm×250 mm,5 μm),以甲醇-0.1%三氟乙酸-乙腈溶液作为流动相进行梯度淋洗,流速1.0 mL/min,在检测波长365 nm,柱温25 ℃条件下,对莨菪亭、芦丁和槲皮素分离检测。方法具有良好的线性关系(r2≥0.999 8)、检出限(≤1.30 μg/L)、回收率(90.67%~113.00%)和重复性(相对标准偏差不大于1.46%,n=8)。该方法准确可靠,可用于定性、定量测定诺丽果粉样品中的莨菪亭、芦丁和槲皮素。

关键词: 超声萃取, 莨菪亭, 芦丁, 槲皮素, 诺丽果粉

Abstract: Noni (Morinda citrifolia) powders are botanical dietary supplement providing purported health benefits, such as scavenging free radicals, anti-cancer activity, and anti-inflammatory effect. This study aimed to optimize the ultrasonic extraction condition and to determine the contents of scopoletin, rutin, and quercetin in noni powders by high performance liquid chromatography (HPLC). The optimized extraction parameters that provided the maximum extraction efficiency were determined as follows: a mixture of methanol and water as extraction solvent, 80%; extraction time, 30 min; temperature, 20 ℃ and ultrasonic frequency, 45 kHz. With an Elite Hypersil ODS column (4.6 mm × 250 mm,5 μm), the HPLC separation was achieved by a gradient mobile phase composed of acetonitrile, methanol, and H2O with a flow rate of 1.0 mL/min at a column temperature of 25 ℃. The quantitative analysis of scopletin, rutin, and quercetin exhibited a good linear relationship, r2≥0.999 8, low detection limit (≤ 1.30 μg/L), high recovery (90.67%–113.00%), and good repeatability (RSD ≤ 1.46%, n = 8). The method proved to be accurate reliable, and suitable for the determination of scopletin, rutin and quercetin in noni powders.

Key words: ultrasonic extraction, scopoletin, rutin, quercetin, noni powder

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