食品科学 ›› 2017, Vol. 38 ›› Issue (6): 303-309.doi: 10.7506/spkx1002-6630-201706047

• 安全检测 • 上一篇    下一篇

桑沟湾养殖牡蛎中贝类毒素监测及预警

宿志伟,赵 峰,姜 雪,刘远平,刘志敏,牟伟丽,周德庆   

  1. 1.中国水产科学研究院黄海水产研究所,农业部水产品质量安全风险评估实验室,山东 青岛 266071;2.山东时进检测服务有限公司,山东 荣成 264300;3.蓬莱京鲁渔业有限公司,山东 蓬莱 265600
  • 出版日期:2017-03-25 发布日期:2017-03-28
  • 基金资助:
    “十二五”国家科技支撑计划项目(2015BAD17B01)

Monitoring and Early Warning of Shellfish Toxins in Oyster Cultured in Sanggou Bay

SU Zhiwei, ZHAO Feng, JIANG Xue, LIU Yuanping, LIU Zhimin, MU Weili, ZHOU Deqing,     

  1. 1. Laboratory of Quality & Safety Risk Assessment for Aquatic Products, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 2. Shandong Shijin Inspection Service Co. Ltd., Rongcheng 264300, China; 3. Penglai Jinglu Fishery Co. Ltd., Penglai 265600, China
  • Online:2017-03-25 Published:2017-03-28

摘要: 利用高效液相色谱-串联质谱(high performance liquid chromatography-tandem mass spectrometry,HPLCMS/MS)方法监测养殖牡蛎中5 种腹泻性贝类毒素与6 种麻痹性贝类毒素含量变化及分布特征,分别利用HP20大孔型吸附树脂与SP700吸附树脂作为富集树脂,富集养殖海域海水中5 种腹泻性贝类毒素与6 种麻痹性贝类毒素,利用HPLC-MS/MS检测方法分析其中毒素含量,同步监测了养殖海域内牡蛎与海水中毒素含量,探究了两者之间的关系,建立了牡蛎内贝类毒素含量随海水内贝类毒素含量之间的变化规律。结果显示:在该海域内一共监测到OA、DTX-1、GYM、PTX-2 4 种腹泻性贝类毒素与STX、dcSTX两种麻痹性贝类毒素;在整个监控期内,海水中所监测贝类毒素随时间变化呈现先增长,达到峰值后逐渐降低趋势。牡蛎中毒素含量与海水中毒素含量呈正相关关系,即牡蛎内毒素的增长随海水内毒素的增长而增长,但牡蛎内毒素含量的峰值出现时间在海水中毒素含量出现峰值之后,延后时间为14 d。根据固相吸附毒素跟踪技术原理,可以提前14 d预警牡蛎内毒素含量。

关键词: 牡蛎, 树脂, 贝类毒素, 监测, 预警

Abstract: The temporal distribution characteristics of five diarrhetic shellfish toxins (DST) and six paralytic shellfish toxins (PST) in oysters cultured in Sanggou Bay were determined by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). At the same time, the adsorption quantities of both kinds of toxins in their growing waters onto HP20 and SP700 macroporous adsorption resins were measured by HPLC-MS/MS to establish the relationships of DST and PST contents between oysters and their growing waters. The results showed that four DST (OA, DTX-1, GYM and PTX-2) and two PST (STX and dcSTX) were detected from both the oysters and seawater samples. During the monitoring period, the shellfish toxins in the seawater increased firstly, reaching a peak, and then gradually reduced. There was a positive correlation between shellfish toxin contents in oysters and their growing seawater. It is surprising that the peak of shellfish toxin contents appeared for oysters 14 days later than for their growing seawater. Thus, solid phase adsorbent and toxin tracking (SPATT) enables warning of the presence of shellfish toxins in oysters 14 days in advance.

Key words: oysters, resins, shellfish toxins, monitoring, early warning

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