食品科学 ›› 2019, Vol. 40 ›› Issue (1): 149-154.doi: 10.7506/spkx1002-6630-20170627-388

• 营养卫生 • 上一篇    下一篇

魔芋低聚糖对高脂饮食小鼠脂代谢的影响

周中凯1,赵亚丽1,2,杨星月1,2   

  1. 1.天津科技大学食品工程与生物技术学院,天津 300457;2.军事医学科学院卫生学环境医学研究所,天津 300041
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFD0400401-2)

Effect of Konjac Oligosaccharide on Lipid Metabolism in Mice Fed High-Fat Diet

ZHOU Zhongkai1, ZHAO Yali1,2, YANG Xingyue1,2   

  1. 1. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; 2. Institute of Environmental & Operational Medicine, Academy of Military Medicine, Chinese Academy of Military Sciences, Tianjin 300041, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 本实验以雄性昆明小鼠为研究对象,将其随机分为正常饲料组、高脂饮食组和低聚糖干预高脂饮食组,将质量分数3%的魔芋低聚糖添加到高脂饲料中,研究魔芋低聚糖对高脂饮食小鼠的体质量、肝指数、血脂及氧化应激水平的调节作用,并采用聚合酶链式反应法测定相关基因表达情况,对机理进行初步探讨。结果表明:在高脂饮食中添加魔芋低聚糖可显著降低小鼠的体质量、肝质量,并有效改善血脂组分,可以抑制小鼠体内丙二醛的生成,这可能与其具有显著提高机体抗氧化能力有关;低聚糖干预显著上调肉碱棕榈酰转移酶的mRNA表达,表明脂肪分解代谢加快;显著上调低密度脂蛋白受体的mRNA表达,可以降低血浆低密度脂蛋白胆固醇水平。综上所述,魔芋低聚糖的干预可以有效降低肥胖所带来的危险。

关键词: 魔芋低聚糖, 高脂饮食, 血脂, 氧化应激

Abstract: In this study, 3% konjac oligosaccharide was added to a high-fat diet to investigate the effect of konjac oligosaccharide on body mass, liver index, lipids and high-fat diet induced oxidative stress in mice. Reverse transcription-polymerase chain reaction (RT-PCR) was adopted to measure gene expression levels to explore the underlying mechanism. Male Kunming mice were randomly divided into normal diet, high-fat diet and oligosaccharide intervention groups. The results showed that the dietary addition of konjac oligosaccharide significantly reduced body mass and liver mass, improved blood lipid profile, and inhibited the generation of malondialdehyde in mice, which may be related to its ability to improve antioxidant capacity in the body. In addition, konjac oligosaccharide could significantly up-regulate the mRNA expression of carnitine palmityl transferase (CPT), thereby accelerating lipid decomposition and metabolism. It could also significantly up-regulate the mRNA expression of low density lipoprotein receptor (LDL-R) and reduce plasma low density lipoprotein cholesterol level. In summary, these results demonstrated that konjac oligosaccharide can effectively reduce the health risks caused by obesity.

Key words: konjac oligosaccharide, high-fat diet, blood lipid, oxidative stress

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