食品科学 ›› 2019, Vol. 40 ›› Issue (1): 177-182.doi: 10.7506/spkx1002-6630-20170711-165

• 营养卫生 • 上一篇    下一篇

发芽糙米米糠营养成分和γ-氨基丁酸分析及缓解体力疲劳功效

王嘉怡1,2,潘姝璇2,3,夏 陈2,邓海云1,2,吕晓华1,*,陈 建2,*   

  1. 1.四川大学华西公共卫生学院,四川 成都 610041;2.四川省农业科学院农产品加工研究所,四川 成都 610066;3.四川农业大学食品学院,四川 雅安 625014
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    四川省财政创新能力提升工程项目(2016TSCY-012);四川省农业科学院条件建设专项-院人才项目

Nutritional Composition, Gamma-Aminobutyric Acid Content and Anti-fatigue Activity of Germinated Brown Rice Bran

WANG Jiayi1,2, PAN Shuxuan2,3, XIA Chen2, DENG Haiyun1,2, Lü Xiaohua1,*, CHEN Jian2,*   

  1. 1. West China School of Public Health, Sichuan University, Chengdu 610041, China; 2. Institute of Agro-products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 3. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 目的:测定发芽糙米米糠(germinated brown rice bran,GBRB)中营养成分及重金属的含量,建立高效液相色谱(high performance liquid chromatography,HPLC)法测定GBRB中γ-氨基丁酸(gamma-aminobutyric acid,GABA),并研究GBRB的抗疲劳功效。方法:1)采用邻苯二甲醛柱前衍生、HPLC法测定GBRB中GABA的含量;2)50 只SD大鼠按体质量随机分为5 组,即空白对照组、高脂对照组和GBRB质量分数分别为30%、20%、10%的高、中、低3 个剂量组。喂饲83 d,进行负重游泳实验,记录游泳力竭时间。结果:1)GBRB的粗纤维、灰分质量分数分别不到普通米糠的29%、33%,粗蛋白、总氨基酸及单一氨基酸(除胱氨酸)、粗脂肪与普通米糠中的含量相近,无机元素含量都低于普通米糠,重金属含量在GB 2762—2012《食品安全国家标准 食品中污染物限量》安全食用限量以下;2)用实验建立的HPLC条件检测GABA,在68.7~275.0 μg/mL范围内线性关系良好,R2为0.999 9,测出的GABA含量为53 mg/100 g,平均回收率为99.61%;3)与空白对照组相比,GBRB高剂量组大鼠游泳力竭时间显著延长(P<0.05)。结论:GBRB的营养成分含量较发芽前有所变化,GABA含量增加,食用高剂量GBRB有利于延长大鼠负重游泳力竭时间,说明GBRB具有缓解体力疲劳的功效。

关键词: 发芽糙米米糠, 营养成分, γ-氨基丁酸, 高效液相色谱, 抗体力疲劳

Abstract: Objective: To determine the contents of nutrients and heavy metals in germinated brown rice bran (GBRB) and establish a high performance liquid chromatography (HPLC) method to detect gamma-aminobutyric acid (GABA) in GBRB, as well as to evaluate the anti-fatigue activity of GBRB. Methods: The content of GABA was determined by HPLC with pre-column o-phthalaldehyde derivatization. Fifty male SD rats were randomly divided into five groups: control, high-fat diet and low-dose (10%), medium-dose (20%), high-dose (30%) GBRB groups. After feeding for 83 days, the rats were forced to swim until exhausted, and the weight-loaded swimming time was recorded. Results: The crude fiber and ash contents in GBRB were nearly 29% of those in ungerminated brown rice bran, although both had similar contents of crude protein, total amino acids and individual amino acids (except for cystine). In addition, the contents of all investigated inorganic elements were lower in GBRB, and the contents of all heavy metals were below the safe limits set in the national standard (GB 2762?2012). Our HPLC method presented good linearity over the concentration range of 68.7–275.0 μg/mL with correlation coefficient (R2) of 0.999 9. The GABA content in GBRB was determined to be 53 mg/100 g. The average recovery of this method was 99.61%. Compared with the control group, high-dose GBRB significantly prolonged the exhaustive swimming time (P < 0.05). Conclusion: The contents of nutrients in brown rice bran change and the GABA content increases after germination. High-dose consumption of GBRB helps prolong the exhaustive swimming time of rats, indicating the anti-fatigue activity of GBRB.

Key words: germinated brown rice bran, nutritional composition, gamma-aminobutyric acid, high performance liquid chromatography, anti-fatigue

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