咖啡碱和绿原酸对高脂饮食小鼠体质量、脂类沉积及肝脏脂质代谢基因表达的影响

doi:10.7506/spkx1002-6630-201709026

摘要/Abstract

摘要： 研究咖啡主要成分咖啡碱和绿原酸对高脂饮食诱导的肥胖小鼠体质量、脂类沉积及肝脏脂类代谢基因表达的影响。50 只雌性ICR小鼠被随机分成5 组：对照组、高脂组、咖啡碱、绿原酸、咖啡碱＋绿原酸，给药组分别在饮水中添加0.05%咖啡碱、0.2%绿原酸、0.05%咖啡碱＋0.2%绿原酸，饲养14 周。饲养期间每周测1 次体质量。饲养结束后心脏采血，摘取脏器和腹腔内脂肪（intraperitoneal adipose tissues，IPAT）并称质量。测定血糖浓度、血中及肝脏中脂质含量。通过实时定量聚合酶链式反应测定肝脏中脂质代谢相关基因AMPK、HMG-CoAr、FASN、ACO的mRNA表达量。与高脂组相比，咖啡碱＋绿原酸能明显抑制小鼠体质量和IPAT质量的增加；咖啡碱、咖啡碱＋绿原酸明显降低血糖和总胆固醇浓度；绿原酸投喂小鼠血中低密度脂蛋白胆固醇浓度显著降低，而血中游离脂肪酸浓度上升；咖啡碱、咖啡碱＋绿原酸显著降低肝脏中总胆固醇和甘油三酯的含量；咖啡碱和咖啡碱＋绿原酸使AMPK基因相对表达量显著上升，FASN基因相对表达量显著下降；3 组给药组的ACO基因相对表达量显著上升，HMG-CoAr基因相对表达量显著下降。咖啡碱＋绿原酸的减肥作用可能通过调节肝脏脂质代谢相关基因的表达，来降低血中和肝脏中脂类的含量，抑制脂肪沉积，抑制小鼠体质量增加，且它们有协同作用。

关键词: 咖啡碱, 绿原酸, 脂肪沉积, 脂类代谢, 基因表达

Abstract: This study was undertaken to investigate the effects of caffeine and chlorogenic acid (CGA), two major components in coffee, on body weight, lipid accumulation and the expression of lipid metabolism-related genes in obese mice fed a high-fat diet. With this aim, 50 female ICR mice were randomly divided into five groups: control, high-fat diet (HFD), caffeine, CGA, and caffeine + CGA groups. The animals in the experimental groups were given drinking water supplemented with 0.05% caffeine, 0.2% CGA and 0.05% caffeine + 0.2% CGA, respectively for 14 consecutive weeks. Body weight was measured daily during the experimental period. Visceral organs and intraperitoneal adipose tissues (IPAT) were harvested and weighed at the end of the experimental period, and blood samples from the heart were collected for measurement of serum glucose and lipid concentrations and liver lipid levels. The mRNA expression levels of lipid metabolism-related genes in liver (AMPK, HMG-CoAr, FASN, and ACO) were determined by real time-quantitative PCR (RT-qPCR). Compared with the HFD group, caffeine + CGA remarkably reduced body weight gain and IPAT weight. The serum glucose and TC levels were decreased significantly by caffeine, and caffeine + CGA. The serum low-density lipoprotein cholesterol (LDL-C) concentration was remarkably lower in mice administrated with CGA, while CGA could result in an increase in serum free fatty acid concentration. Caffeine alone and in combination with CGA remarkably reduced the liver total cholesterols (TC) and triglyceride (TG) levels, significantly increased the mRNA expression of AMPK and significantly reduced the mRNA expression of FASN in liver. A significant up-regulation in the mRNA expression of ACO and a significant down-regulation in the mRNA expression of HMG-CoAr in liver were observed for all three experimental groups. Conclusions: The anti-obesity effects of caffeine + CGA may be achieved through regulating the mRNA expression of lipid metabolism-related genes in liver, modulating serum and liver lipid levels in, and inhibiting body weight gain and fat accumulation in mice in a synergistic manner.

Key words: caffeine, chlorogenic acid, fat accumulation, lipid metabolism, mRNA expression

中图分类号:

TS201.4

朱艳萍，杨丽聪，林乐珍，淦述翔，郑国栋. 咖啡碱和绿原酸对高脂饮食小鼠体质量、脂类沉积及肝脏脂质代谢基因表达的影响[J]. 食品科学, 2017, 38(9): 162-167.

ZHU Yanping, YANG Licong, LIN Lezhen, GAN Shuxiang, ZHENG Guodong. Effect of Caffeine and Chlorogenic Acid on Body Weight, Lipid Accumulation and the Expression of Lipid Metabolism-Related Genes in High-Fat Diet-Fed Mice[J]. FOOD SCIENCE, 2017, 38(9): 162-167.

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咖啡碱和绿原酸对高脂饮食小鼠体质量、脂类沉积及肝脏脂质代谢基因表达的影响

Effect of Caffeine and Chlorogenic Acid on Body Weight, Lipid Accumulation and the Expression of Lipid Metabolism-Related Genes in High-Fat Diet-Fed Mice

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