食品科学 ›› 2019, Vol. 40 ›› Issue (1): 183-189.doi: 10.7506/spkx1002-6630-20170917-242

• 营养卫生 • 上一篇    下一篇

云南小粒咖啡类黑精的抗氧化及减肥功能

王 瑶,王晓娜,张雪辉,殷建忠,潘红梅,吴志霜,冯月梅,吴少雄*,王松梅*   

  1. 昆明医科大学公共卫生学院,云南 昆明 650500
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    云南省高校重点实验室建设项目(YNHEKL201608);昆明医科大学科技创新团队建设项目(CXTD201603);昆明医科大学重大科技成果培育项目(CGPY201605)

Antioxidant and Antiobesity Effects of Melanoidins from Yunnan Grown Arabica

WANG Yao, WANG Xiaona, ZHANG Xuehui, YIN Jianzhong, PAN Hongmei, WU Zhishuang, FENG Yuemei, WU Shaoxiong*, WANG Songmei*   

  1. College of Public Health, Kunming Medical University, Kunming 650500, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 目的:探讨云南小粒咖啡类黑精的抗氧化能力和减肥功能。方法:用热水提取法提取中烘焙度云南小粒咖啡中的类黑精(M总),再将提取的类黑精产物按分子质量大小分成>100 kDa(M1)、10~100 kDa(M2)、<10 kDa(M3)3 个部分。用羟自由基、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率和总还原力评价M总、M1、M2、M3的抗氧化能力;通过饲喂高脂饲料建立大鼠肥胖模型,检测不同质量浓度咖啡类黑精对肥胖大鼠体质量、体脂质量、血脂水平、脂体比、脂肪组织形态、肝脏组织形态等指标的影响。结果:相同质量浓度下,云南小粒咖啡类黑精不同分子质量产物自由基清除能力和总还原力不同;不同剂量处理组咖啡类黑精均能降低大鼠体质量、体脂质量;肝组织切片显示,中、高剂量处理组大鼠肝脏组织结构完整、肝细胞大小一致、胞浆均匀、细胞核清晰;脂肪组织切片显示,高剂量处理组大鼠脂肪细胞较模型组减小,且排列均匀、结构完整。结论:云南小粒咖啡类黑精及不同分子质量产物均具有不同程度抗氧化能力;高质量浓度(72 g/100 mL)云南小粒咖啡类黑精具有一定的减肥作用,能抑制大鼠体质量的增加,减少肝脏内脂肪堆积,对肝脏具有一定的保护作用。

关键词: 云南小粒咖啡, 类黑精, 抗氧化作用, 减肥功能

Abstract: Objective: To study the antioxidant and antiobesity effects of melanoidin from Yunnan grown arabica. Methods: Total melanoidins from medium roasted arabica were extracted by hot water extraction method, and then were fractionated into three fractions: > 100 kDa (M1), 10-100 kDa (M2) and < 10 kDa (M3) by ultrafiltration. The antioxidant capacity of four samples was determined by hydroxyl radical scavenging, 1,1-diphenyl-2-picrylhydrazyl radical scavenging and total reducing power assays. The effects of the total melanoidins at different concentrations on body mass, body fat content, blood lipid levels, body fat ratio, adipose tissue morphology and liver tissue morphology in a rat model of high-fat dietinduced obesity were determined. Results: All melanoidin samples had antioxidant activity varying with molecular mass at identical concentrations. The total melanoidins at all doses reduced body mass and body fat content in the obese rats. Histological examination showed that the liver tissue structure in the melanoidin treated rats at medium and high doses remained intact, accompanied by homogeneous hepatocytes and cytoplasm as well as clear nuclei. In addition to being intact and orderly arranged, the hepatocytes of the high-dose treated rats were smaller than those of the model group. Conclusion: The total melanoidins and three fractions from Yunnan grown arabica all had strong antioxidant capacity. The total melanoidins had an antiobesity effect by inhibiting body mass gain and reducing fat accumulation in the liver and consequently protecting the liver.

Key words: Yunnan grown arabica, melanoidins, antioxidant effect, antiobesity effect

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