食品科学 ›› 2019, Vol. 40 ›› Issue (5): 197-201.doi: 10.7506/spkx1002-6630-20171021-232

• 营养卫生 • 上一篇    下一篇

甘薯汁对果蝇寿命及抗氧化能力的影响

张碧莹,钱籽霖,唐 玲,张 静,蒋和体*   

  1. 西南大学食品科学学院,重庆 400715
  • 出版日期:2019-03-15 发布日期:2019-04-02
  • 基金资助:
    重庆市科委社会事业与民生保障科技创新专项(cstc2015shms-ztzx80013)

Effect of Sweet Potato Juice on Life Span and Antioxidant Capacity in Drosophila melanogaster

ZHANG Biying, QIAN Zilin, TANG Ling, ZHANG Jing, JIANG Heti*   

  1. College of Food Science, Southwest University, Chongqing 400715, China
  • Online:2019-03-15 Published:2019-04-02

摘要: 为探讨甘薯汁抗氧化及防衰老的功效,选取果蝇为动物模型,在培养基中分别添加低、中、高剂量(分别为0.10、0.15、0.20 mg/mL)甘薯汁饲喂8 h内羽化未交配的雌、雄果蝇,通过果蝇生存实验及测定不同饲养时间果蝇体内的超氧化物歧化酶(superoxide dismutase,SOD)活力、总抗氧化能力(total antioxidant capacity,T-AOC)和丙二醛(malondialdehyde,MDA)含量,评价甘薯汁对果蝇寿命和抗氧化酶活性的影响。结果表明:与对照组相比,不同剂量甘薯汁组均能显著延长雌、雄果蝇的平均寿命(P<0.05或P<0.01),且呈剂量依赖性。果蝇SOD活力、T-AOC在低、中、高剂量组中均显著增加(P<0.05或P<0.01),中、高剂量组果蝇体内MDA含量显著降低(P<0.05或P<0.01)。其中,与对照组相比,高剂量组可使雌、雄果蝇平均寿命分别延长17.93%和16.00%,SOD活力分别提高50.51%、58.61%,T-AOC分别提高79.73%、62.67%,MDA含量分别下降35.59%、37.14%。结论:甘薯汁可以提高果蝇体内的抗氧化能力,具有一定的延缓衰老作用。

关键词: 甘薯汁, 抗衰老, 寿命, 果蝇

Abstract: In order to investigate the antioxidant and anti-aging effect of sweet potato juice, Drosophila melanogaster was chosen as an animal model. Male and female Drosophila melanogaster with eclosion and without mating within 8 h were fed on a diet supplemented with low, moderate or high doses (0.10, 0.15, and 0.20 mg/mL) of sweet potato juice. The survival rate of D. melanogaster was investigated, and the activity of superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content were measured in its homogenate. The results showed that compared with the control group, average life span and maximum life span in all dose groups were significantly increased (P < 0.05 or P < 0.01) in a dose-dependent manner. SOD activity and T-AOC were significantly increased in all dose groups (P < 0.05 or P < 0.01), whereas MDA content was significantly decreased in the middle- and high-dose groups (P < 0.05 or P < 0.01). Compared with the control group, average life span in the high-dose group was extended by 17.93% and 16.00% for female and male flies, the activity of SOD was increased by 50.51% and 58.61%, T-AOC activity was increased by 79.73% and 62.67%, while the content of MDA was decreased by 35.59% and 37.14%, respectively. In conclusion, sweet potato juice can prolong life span and delay aging in D. melanegaster.

Key words: sweet potato juice, senescence resistance, life span, Drosophila melanogaster

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