食品科学 ›› 2017, Vol. 38 ›› Issue (11): 155-160.doi: 10.7506/spkx1002-6630-201711025

• 基础研究 • 上一篇    下一篇

桉叶精油包埋前后抑菌性能及成分比较研究

岳淑丽,任小玲,陈 霞,向 红,孙远明,凌舒琦,刘荟雅   

  1. 华南农业大学食品学院,广东 广州 510642
  • 出版日期:2017-06-15 发布日期:2017-06-19

Comparative Study of Antimicrobial Activity and Composition of Eucalyptus Essential Oil before and after Encapsulation

YUE Shuli, REN Xiaoling, CHEN Xia, XIANG Hong, SUN Yuanming, LING Shuqi, LIU Huiya   

  1. College of Food Science, South China Agricultural University, Guangzhou 510642, China
  • Online:2017-06-15 Published:2017-06-19

摘要: 以包埋前后的桉叶精油为研究对象,采用平板打孔法和气相扩散法分别测定其对供试菌的抑菌活性和气相最低抑菌浓度(minimum inhibitory concentration,MIC)、气相最低杀菌浓度(minimum bactericidal concentration,MBC),探讨桉叶精油包埋前后抑菌性能差异。并用气相色谱-质谱联用技术对比分析桉叶精油包埋前后的化学成分。结果表明:桉叶精油包埋前后对供试细菌、酵母菌和霉菌均有一定的抑制作用。对酵母菌的抑制作用最强,其次为细菌,对霉菌的抑制效果稍差。桉叶精油包埋前后对供试菌的气相MIC和MBC未有太大差异。桉叶精油包埋前后主要成分大体相同,均以萜烯类和醇类化合物为主。包埋后桉叶精油中部分萜烯类化合物的消失或相对含量的减少导致抑菌性能稍有下降,但气相MIC不变,仅对酿酒酵母和桔青霉的气相MBC稍有上升。

关键词: 桉叶精油, 包埋, 抑菌性能, 气相扩散, 化学成分

Abstract: The antibacterial activity of encapsulated and non-encapsulated eucalyptus essential oil (EEO) was determined by the agar well diffusion method, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were studied by vapor diffusion method. Besides, the chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the EEO had antibacterial activity against all tested bacteria, yeast and mold irrespective of encapsulation. Its antimicrobial activity against yeast was the strongest followed by bacteria, and its antimold activity was weaker. The MIC and MBC of EEO did not significantly differ before and after encapsulation, and the major chemical components were identical, including terpenes and alcohols. The disappearance or decrease of some terpenes in the essential oil after encapsulation resulted in a slight decrease in its antibacterial activity; while its MIC remained unchanged, its MBC against Saccharomyces cerevisiae and Penicillium citrinum was slightly increased.

Key words: eucalyptus essential oil, encapsulation, antibacterial properties, vapor diffusion, chemical composition

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