β-胡萝卜素对H2O2诱导的斑马鱼肝损伤的保护作用

doi:10.7506/spkx1002-6630-20171117-217

食品科学 ›› 2019, Vol. 40 ›› Issue (5): 162-166.doi: 10.7506/spkx1002-6630-20171117-217

β-胡萝卜素对H2O2诱导的斑马鱼肝损伤的保护作用

曲蕙名1,2，王莹1，赵博1，刘建龙2，刘可春1，沈红1，楚杰1,*

1.齐鲁工业大学（山东省科学院）生物研究所，山东济南 250103；2.山东师范大学生命科学学院，山东济南 250014

出版日期:2019-03-15 发布日期:2019-04-02
基金资助:
国家国际科技合作项目（2014DFR30840）；国家重点研发计划-国际科技创新合作重点专项（2016YFE0111600）；山东省科学院青年基金项目（2018QN0025）

Protective Effect of β-Carotene on Liver Oxidative Damage Induced by Hydrogen Peroxide in Zebrafish

QU Huiming1,2, WANG Ying1, ZHAO Bo1, LIU Jianlong2, LIU Kechun1, SHEN Hong1, CHU Jie1,*

1. Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China; 2. Life Sciences Institute of Shandong Normal University, Jinan 250014, China

Online:2019-03-15 Published:2019-04-02

摘要/Abstract

摘要： 研究β-胡萝卜素对H2O2诱导的斑马鱼肝损伤的保护作用。选择适宜浓度的H2O2诱导斑马鱼肝脏氧化应激损伤，分别以5、10、20 μg/mL的β-胡萝卜素对其进行保护。给药48 h后检测组织匀浆中谷丙转氨酶（alanine aminotransferase，ALT）、谷草转氨酶（aspartate aminotransferase，AST）、超氧化物歧化酶（superoxide dismutase，SOD）活力和丙二醛（malondialdelyde，MDA）含量；苏木精-伊红染色观察斑马鱼肝组织病理变化情况；实时荧光定量聚合酶链式反应技术检测SOD、CAT和GSTP2以及核转录因子Nrf2 mRNA的相对表达量。结果表明：H2O2处理斑马鱼仔鱼48 h后，组织匀浆液中ALT和AST活力升高，SOD活力降低，MDA含量升高；与模型组比较，β-胡萝卜素能够抑制ALT和AST的升高，提高SOD活力，降低MDA含量；病理切片结果显示β-胡萝卜素组能显著改善斑马鱼肝脏组织病理形态；提高Nrf2 mRNA的相对表达量，上调抗氧化酶基因的相对表达量。β-胡萝卜素对H2O2诱导的斑马鱼肝损伤具有保护作用。

关键词: β-胡萝卜素, H2O2, 斑马鱼, 肝损伤

Abstract: The protective effect of β-carotene on H2O2-induced liver injury in zebrafish was investigated. Liver injury model was induced by H2O2 at the proper concentration, and β-carotene at doses of 5, 10 and 20 μg/mL was used to suppress H2O2-induced damage. After 48 h of incubation, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and superoxide dismutase (SOD) activities and malondialdelyde (MDA) content were measured. Pathological changes in the liver were observed by hematoxylin-eosin (HE) staining. The relative mRNA expression levels of SOD, CAT, GSTP2 and Nrf2 were assessed by real-time quantitative polymerase chain reaction (qPCR). The results showed that H2O2 elevated the levels of ALT, AST and MDA and reduced the level of SOD. Compared with the model group, β-carotene decreased the levels of ALT, AST, and MDA and improved the activity of SOD. Moreover, β-carotene significantly ameliorated hepatic pathological changes and upregulated the relative mRNA expression levels of SOD, CAT, GSTP2 and Nrf2. Finally we conclude that β-carotene has a protective effect on liver injury induced by H2O2 in zebrafish.

Key words: β-carotene, H2O2, zebrafish, liver injury

中图分类号:

Q593.1

曲蕙名，王莹，赵博，刘建龙，刘可春，沈红，楚杰. β-胡萝卜素对H2O2诱导的斑马鱼肝损伤的保护作用[J]. 食品科学, 2019, 40(5): 162-166.

QU Huiming, WANG Ying, ZHAO Bo, LIU Jianlong, LIU Kechun, SHEN Hong, CHU Jie. Protective Effect of β-Carotene on Liver Oxidative Damage Induced by Hydrogen Peroxide in Zebrafish[J]. FOOD SCIENCE, 2019, 40(5): 162-166.

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β-胡萝卜素对H2O2诱导的斑马鱼肝损伤的保护作用

Protective Effect of β-Carotene on Liver Oxidative Damage Induced by Hydrogen Peroxide in Zebrafish

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