食品科学 ›› 2017, Vol. 38 ›› Issue (12): 292-297.doi: 10.7506/spkx1002-6630-201712045

• 安全检测 • 上一篇    下一篇

用于农残快速检测的离心式微流控芯片研制

叶嘉明,邵佳美,杨平,范真真,冯何凯,陈波,杜汉根   

  1. 1.浙江清华长三角研究院萧山生物工程中心,浙江?杭州 311231;2.国家食品安全风险评估中心应用技术合作中心,浙江?嘉兴 314006;3.深圳出入境检验检疫局食品检验检疫技术中心,广州?深圳 518045;4.浙江工商大学食品与生物工程学院,浙江?杭州 310018
  • 出版日期:2017-06-25 发布日期:2017-06-26
  • 基金资助:
    食品科学与工程浙江省重中之重一级学科开放基金项目(JYTSP20142062);嘉兴市公益性应用技术研究计划项目(2015AY23006)

Fabrication and Application of Centrifugal Microfludic Chip for Rapid Detection of Pesticide Residues

YE Jiaming, SHAO Jiamei, YANG Ping, FAN Zhenzhen, FENG Hekai, CHEN Bo, DU Hangen   

  1. 1. Biotechnology Center at Xiaoshan, Yangtze Delta Region Institute of Tsinghua University, Hangzhou 311231, China; 2. Cooperation Center for Application Technology, China National Center for Food Safety Risk Assessment, Jiaxing 314006, China; 3. Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen 518045, China; 4. School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China
  • Online:2017-06-25 Published:2017-06-26

摘要: 基于酶抑制原理结合光度分析方法,研制一种预存储生化试剂的离心式微流控芯片。设计制作的一次性微流控CD芯片集成进样、酶抑制反应、显色反应及检测单元,结合自行研制的便携式分析装置,可以同时检测12?个样品,能够实现对大批量农产品中有机磷和氨基甲酸酯类农药残留进行现场、快速、高通量检测。结果表明:与传统农残快速测定仪比较,微流控芯片农残速测系统操作单元集成度高,可以实现农残检测流程的自动化,样品及试剂消耗量降至传统速测方法的约1/20,同时检测灵敏度、重复性和准确性整体优于传统农残快速测定仪,可以满足基层非专业人员针对大批量样品农药残留的筛查需求。

关键词: 微流控芯片, 农药残留, 快速检测, 酶抑制法

Abstract: In this paper, a novel disposable centrifugal microfludic chip with pre-storage biochemical reagents was developed for the rapid detection of pesticide residues in agricultural products, which was based on the principle of enzyme inhibition and photometric analysis. The microfludic chip was integrated with sampling, enzyme inhibition, color development and detection chamber. By coupling with a portable detection device developed in our laboratory, the microfludic chip could provide a simple, easy-to-use, low-cost and sensitive approach for rapid detection of organophosphate and carbamate pesticides on site. Results indicated that the microfluidic chip could achieve an automatic detection process with the advantages of less consumption of reagents (reduction by one-twentieth) and satisfied accuracy compared with the traditional pesticide residue detector. Furthermore, the microfluidic chip was proved to be a powerful tool for screening pesticide residues in the case of high-throughput requirement. The microfluidic chip was superior to the traditional pesticide residue detector in term of detection sensitivity, reproducibility and measurement accuracy. The microfluidic chip coupled with self-made detection device is particularly suitable for on-site, rapid and high-throughput screening of pesticide residues by nonprofessionals.

Key words: microfluidic chip, pesticide residues, rapid detection, acetylcholin esterase (Ache) inhibition method

中图分类号: