黑灵芝多糖体内抗炎活性及对甘露糖受体表达的影响

doi:10.7506/spkx1002-6630-201713028

摘要/Abstract

摘要： 目的：研究水溶性黑灵芝多糖（a water-soluble polysaccharides from Ganoderma atrum，PSG）-1体内抗炎活性及对甘露糖受体（mannose receptor，MR）表达的影响。方法：腹腔注射脂多糖（lipopolysaccharide，LPS）建立小鼠体内炎症模型，随机分为5 组：正常对照组、LPS组、PSG-1（高、中、低剂量，分别为100、50、25 mg/（kg·d））＋LPS组。流式细胞仪检测巨噬细胞中MR表达、吞噬功能及活性氧（reactiveoxygen species，ROS）生成，用酶联免疫吸附测定（enzyme-linked immunosorbent assay，ELISA）分析血清中肿瘤坏死因子（tumor necrosis factor，TNF）-α、白细胞介素（interleukin，IL）-1β和IL-6的含量。结果：与正常对照组相比，L P S组腹腔巨噬细胞表面MR表达量下降，与L P S组相比，P S G - 1＋L P S组腹腔巨噬细胞表面MR表达量极显著增加（P＜0.01）；与正常对照组相比，L P S组腹腔巨噬细胞吞噬能力和ROS生成增加，与LPS组相比，PSG-1＋LPS组腹腔巨噬细胞吞噬功能和ROS生成显著降低（P＜0.05，P＜0.01）；与正常对照组相比，小鼠经L P S处理后，血清中T N F - α、I L - 1 β和IL-6的分泌水平极显著升高（P＜0.01）；与LPS组相比，小鼠灌胃PSG-1后，PSG-1（高剂量）＋LPS组中TNF-α的含量显著降低（P＜0.05），IL-1β和IL-6的分泌水平无显著差异。结论： PSG-1具有抗炎作用，可部分抑制LPS诱导的体内炎症，其机理与PSG-1促进小鼠腹腔巨噬细胞表面MR的表达、抑制巨噬细胞向M1型极化有关。

关键词: 黑灵芝多糖, 脂多糖, 巨噬细胞, 甘露糖受体, 吞噬功能, 炎症因子, 极化

Abstract: Objective: To explore the in vivo anti-inflammatory activity of a water-soluble polysaccharide purified from Ganoderma atrum, named PSG-1, and its effect on mannose receptor (MR) expression. Methods: In the present study, lipopolysaccharide (LPS) via intraperitoneal injection was applied to establish a mouse model of inflammation. Mice were randomly divided into five groups: control group, LPS group, high-dose PSG-1 (100 mg/(kg·d)) + LPS group, mediumdose PSG-1 (50 mg/(kg·d)) + LPS group and low-dose PSG-1 (25 mg/(kg·d)) + LPS group. MR expression, phagocytosis and the level of reactive oxygen species (ROS) in macrophages were analyzed by flow cytometry. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in serum were determined by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the control group, MR expression on the macrophage surface was decreased in the LPS group. In contrast, MR expression on the macrophage surface was significantly increased in the PSG-1 + LPS group in comparison with the LPS group (P < 0.01). Macrophage phagocytosis and the level of ROS in the LPS group were higher than in the control group, which were significantly decreased after administration of PSG-1 (P＜0.05, P＜0.01). After mice were administrated with LPS, the levels of TNF-α, IL-1β and IL-6 were significantly increased in serum in comparison with the control group (P < 0.01). Compared with the LPS group, the expression level of TNF-α was significantly decreased after administration of high-dose PSG-1 (P < 0.05), but the expression levels of IL-1β and IL-6 did not significantly change. Conclusion: PSG-1 had an anti-inflammatory effect, being able to partly suppress inflammation induced by LPS in vivo, by promoting MR expression on the peritoneal macrophage surface in mice and suppressing macrophage polarization to M1 phenotype.

Key words: Ganoderma atrum polysaccharide, lipopolysaccharide, macrophage, mannose receptor, phagocytosis, cytokine, polarization

中图分类号:

TS201.4

张妍淞，李文娟，汤小芳，聂少平，谢明勇. 黑灵芝多糖体内抗炎活性及对甘露糖受体表达的影响[J]. 食品科学, 2017, 38(13): 167-173.

ZHANG Yansong, LI Wenjuan, TANG Xiaofang, NIE Shaoping, XIE Mingyong. In Vivo Anti-Inflammatory Activity of a Polysaccharide from Ganoderma atrum and Its Effect on Mannose Receptor Expression[J]. FOOD SCIENCE, 2017, 38(13): 167-173.

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黑灵芝多糖体内抗炎活性及对甘露糖受体表达的影响

In Vivo Anti-Inflammatory Activity of a Polysaccharide from Ganoderma atrum and Its Effect on Mannose Receptor Expression

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