食品科学 ›› 2017, Vol. 38 ›› Issue (14): 112-119.doi: 10.7506/spkx1002-6630-201714017

• 生物工程 • 上一篇    下一篇

具有菲环骨架的高分子载体固定淀粉酶交联聚集体

黎克纯,卢建芳,周菊英,,许海棠,,赵彦芝,   

  1. (1.广西民族大学化学化工学院,广西?南宁 530006;2.广西林产化学与工程重点实验室,广西?南宁 530006;3.广西高校协同创新中心,广西?南宁 530006)
  • 出版日期:2017-07-25 发布日期:2017-09-06
  • 基金资助:
    国家民委项目(14GXZ012);广西高校科学技术研究项目(KY2015LX069;KY2015YB080);广西民族大学科研项目(2016MDQN019;2016MDYB025)

Immobilization of Cross-Linked Amylase Aggregates on Polymer Containing Phenanthrene Skeleton

LI Kechun, LU Jianfang,, ZHOU Juying, XU Haitang, ZHAO Yanzhi,   

  1. (1. School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China; 2. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Nanning 530006, China; 3. Collaborative Innovation Center in Guangxi, Nanning 530006, China)
  • Online:2017-07-25 Published:2017-09-06

摘要: 用80%异丙醇溶液将淀粉酶沉淀聚集,随后用戊二醛交联制备交联淀粉酶聚集体(cross-linked amylase aggregates,CLEAs-A),最后将CLEAs-A共价固定在具有大孔的菲环骨架的载体上,制备固定化CLEAs-A。探讨各种优化条件,研究固定化CLEAs-A的酶学性质,结果显示固定化酶的热稳定性和储存稳定性明显提高,重复反应7 批次后,相对活性仍保留63.29%。此外,扫描电子显微镜和孔径测量展现了固定化CLEAs-A的表面和孔径结构。此法可作为一种通用方法,制备出工业生产中所需要的具有高生物催化性能的固定化酶。

关键词: 淀粉酶, 交联酶淀粉酶聚集体(CLEAs-A), 固定化CLEAs-A, 菲环骨架

Abstract: Amylase was precipitated with 80% isopropanol followedcross-linking with glutaraldehyde to obtain cross-linked amylase aggregates (CLEAs-A). Then CLEAs-A was covalently immobilized on a macroporous polymer carrier containing a phenanthrene skeleton to obtain immobilized CLEAs-A. Herein, various process parameters were systematically evaluated. Moreover, the enzymatic properties and physical structure of immobilized CLEAs-A were investigated. The thermal and storage stability were improved remarkably as compared with those of the free amylase. After seventh repeated use, the activity recovery of immobilized CLEAs-A was still as high as 63.29%. Furthermore, scanning electron microscopy and porosity measurements indicated the surface and pore structure of immobilized CLEAs-A. The proposed immobilization strategy would provide a general approach for preparing immobilized enzymes with robust and high bio-catalytic properties for in industrial production.

Key words: amylase, cross-linked amylase aggregates (CLEAs-A), immobilized CLEAs-A, phenanthrene skeleton

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