食品科学 ›› 2017, Vol. 38 ›› Issue (14): 170-174.doi: 10.7506/spkx1002-6630-201714026

• 成分分析 • 上一篇    下一篇

HPLC-ELSD法同时测定鲜枣果实中不同种类可溶性糖含量

蒲小秋,白红进,马倩倩,吴翠云   

  1. (1.塔里木大学植物科学学院,新疆生产建设兵团南疆特色果树生产工程实验室,新疆?阿拉尔 843300;2.塔里木大学 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆?阿拉尔 843300)
  • 出版日期:2017-07-25 发布日期:2017-09-06
  • 基金资助:
    浙江大学馥莉食品研究院基金项目(KY201004);兵团科技创新团队建设项目(2014CC006); 新疆研究生科研创新项目(XJGRI2016128)

Simultaneous Determination of Water-Soluble Sugars in Fresh Jujube by High Performance Liquid Chromatography with Evaporative Light-Scattering Detector (HPLC-ELSD)

PU Xiaoqiu, BAI Hongjin, MA Qianqian, WU Cuiyun   

  1. (1. Engineering Laboratory of Characteristic Fruit Tree Production in Southern Xinjiang of Xinjiang Production and Construction Corps, College of Plant Science, Tarim University, Alar 843300, China; 2. Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, China)
  • Online:2017-07-25 Published:2017-09-06

摘要: 摘 要:采用高效液相色谱-蒸发光散射检测器建立鲜枣果实中不同种类可溶性糖含量的测定方法。结果表明:适宜分离条件为Waters XBridgeTM Amide色谱柱(4.6?mm×250?mm,5?μm),流动相A为0.2%三乙胺的超纯水溶液,流动相B为0.2%三乙胺-乙腈溶液,两相体积比为24∶76,柱温30?℃,雾化管温度60?℃,漂移管温度60℃,气流量1.6?L/min,增益值1.0。在该条件下鼠李糖、阿拉伯糖、果糖、甘露糖、葡萄糖、蔗糖、麦芽糖和乳糖均能得到较好分离(分离度≥1.5),在0.099~1.040?ng/μL范围内呈良好线性关系,8?种糖的加标回收率在93.1%~111.2%之间,相对标准偏差均小于5%;检出限(RSN=3)在0.008~0.03?ng/μL之间。该方法具有操作简便、分离效果好、分离时间短等特点,可用于鲜枣果实中不同种类可溶性糖含量的测定,与传统方法相比较更加灵敏准确,有利于品质鉴定。

关键词: 鲜枣, 高效液相色谱-蒸发光散射检测器, 水溶性糖, 单糖, 低聚糖?

Abstract: A method for the simultaneous determination of water-soluble sugars in fresh jujubes by high performance liquid chromatography with evaporative light-scattering detector (HPLC-ELSD) was established. The carbohydrates were separated on a chromatographic column of Waters XBridgeTM Amide (4.6 mm × 250 mm, 5 μm) with a mobile phase consisting of A (0.2% triethylamine aqueous solution) and B (0.2% triethylamine in acetonitrile) (A:B = 24:76, V/V). The column temperature was 30 ℃ and the atomization temperature was 60 ℃. The drift tube temperature of ELSD was 60 ℃. Nitrogen was used as the carried gas with a flow rate of 1.6 L/min and a gain value of 1.0. These experimental conditions allowed good separation of water-soluble sugars, such as rhamnose, arabinose, glucose, mannose, glucose, sucrose, maltose and lactose with a resolution of 1.5 or above. Good linearity was observed for the analytes in the concentration range of 0.099–1.040 ng/μL. The recoveries of spiked real samples ranged from 93.1% to 111.2%, with relative standard deviation (RSD) values below 5%. The limits of detection (RSN = 3) were in the range of 0.008–0.03 ng/μL. This method had a lot of advantages including simple operation, effective separation and time saving, and could be used to determine water-soluble sugars in fresh jujube. Compared with the traditional spectrophotometric method, HPLC-ELSD was more sensitive and accurate making it more suitable for quality identification of fresh jujube.

Key words: jujube fruit, high performance liquid chromatography with evaporative light-scattering detector (HPLC-ELSD), water-soluble sugar, monosaccharide, oligosaccharide

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