食品科学 ›› 2017, Vol. 38 ›› Issue (17): 8-13.doi: 10.7506/spkx1002-6630-201717002

• 基础研究 • 上一篇    下一篇

体外模拟消化对车前子来源毛蕊花苷抑制黄嘌呤氧化酶活性的影响

万 茵1,张阿惜1,陈雪洋2,陈 明3,余迎利1,夏冬华1,付桂明1,4,*   

  1. 1.南昌大学食品学院,食品科学与技术国家重点实验室,江西 南昌 330047;2.南阳农业职业学院农业科学系,河南 南阳 473000;3.南昌大学第一附属医院,江西 南昌 330006;4.南昌大学中德食品工程中心,江西 南昌 330047
  • 出版日期:2017-09-15 发布日期:2017-09-12
  • 基金资助:
    国家自然科学基金地区科学基金项目(31160316)

Effects of in Vitro Simulated Degradation on the Xanthine Oxidase Inhibitory Activity of Verbascoside from Plantago asiatica L.

WAN Yin1, ZHANG Axi1, CHEN Xueyang2, CHEN Ming3, YU Yingli1, XIA Donghua1, FU Guiming1,4,*   

  1. 1. State Key Laboratory of Food Science and Technology, College of Food Science, Nanchang University, Nanchang 330047, China;2. Department of Agricultural Sciences, Nanyang Vocational College of Agriculture, Nanyang 473000, China;3. The First Affiliated Hospital of Nanchang University, Nanchang 330006, China;4. Sino-German Food Engineering Center, Nanchang University, Nanchang 330047, China
  • Online:2017-09-15 Published:2017-09-12

摘要: 研究体外模拟消化各阶段的条件对车前子来源毛蕊花苷抑制黄嘌呤氧化酶活性的影响。结果发现毛蕊花苷含量在模拟消化作用4 h后降低,降低程度最高的是肠菌孵育样液,毛蕊花苷含量减少率为(71.26±0.96)%,其次是累加消化样液,达(28.46±0.50)%。模拟消化后各样液的黄嘌呤氧化酶抑制作用均有所增加,增加程度最大的为肠菌孵育样液(P<0.05),其次为累加消化样液。经高效液相色谱-质谱联用分析发现,各消化样液中均能检出毛蕊花苷的代谢苷元咖啡酸和羟基酪醇,活性实验表明后两者对黄嘌呤氧化酶的体外抑制活性高于前体毛蕊花苷。毛蕊花苷降解成具有黄嘌呤氧化酶体外抑制活性的产物可能是其在体内表现生理活性的途径之一。

关键词: 毛蕊花苷, 模拟消化, 黄嘌呤氧化酶, 体外抑制作用, 苷元

Abstract: In this study, the effects of different stages of in vitro simulated digestion on the stability of Plantago asiatica L. seed verbascoside (PSV) were explored. It was found that the concentration of PSV in all simulated digestion systems was reduced after digestion for 4 h, and the highest reduction of (71.26 ± 0.96)% was observed in the gut bacterial culture, followed by the simulated gastrointestinal digestion system ((28.46 ± 0.50)%). After simulated digestion, xanthine oxidase inhibitory activity in various simulated digestion systems increased, in particular in the gut bacterial culture (P < 0.05), followed by the simulated gastrointestinal digestion system. The aglycone metabolites of verbascoside, caffeic acid and hydroxytyrosol, were detected from all simulated digestion systems by LC-MS. The in vitro xanthine oxidase inhibitory activity of caffeic acid and hydroxytyrosol was higher than that of verbascoside. The degradation of verbascoside into xanthine oxidase inhibitory metabolites is likely to be one of the effective ways to exert its physiological activity in vivo.

Key words: verbascoside, simulated digestion, xanthine oxidase, in vitro inhibition activity, aglycone

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