巴莲莲子生物碱提取物对CCl4诱导小鼠肝损伤的预防效果

doi:10.7506/spkx1002-6630-201717035

食品科学 ›› 2017, Vol. 38 ›› Issue (17): 216-222.doi: 10.7506/spkx1002-6630-201717035

巴莲莲子生物碱提取物对CCl4诱导小鼠肝损伤的预防效果

冯霞1，孙鹏1，易若琨1，彭德光2，赵欣1,*

1.重庆第二师范学院生物与化学工程学院，重庆市功能性食品协同创新中心，重庆市功能性食品工程技术研究中心，功能性食品研发重庆市工程实验室，重庆 400067；2.重庆市巴莲品种选育及深加工企业工程技术研究中心，重庆 400041

出版日期:2017-09-15 发布日期:2017-09-12
基金资助:
重庆高校创新团队建设计划资助项目（CXTD201601040）；重庆市工程技术研究中心建设项目（cstc2015yfpt_gcjsyjzx0027）；重庆第二师范学院校级科研项目（KY201523B）

Preventive Effects of Alkaloids Extracted from Ba Lotus Seeds on CCl4-Induced Hepatic Injury in Mice

FENG Xia1, SUN Peng1, YI Ruokun1, PENG Deguang2, ZHAO Xin1,*

1. Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Collaborative Innovation Center for Functional Food,College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China;2. Chongqing Enterprise Engineering Research Center of Ba-lotus Breeding and Deep Processing, Chongqing 400041, China

Online:2017-09-15 Published:2017-09-12

摘要/Abstract

摘要： 为检测巴莲莲子生物碱提取物（alkaloids extracted from Ba lotus seed，AEBLS）对CCl4诱导的小鼠肝损伤的预防作用，以732交换树脂分离纯化的AEBLS为研究对象，对小鼠灌胃低、高剂量（50 、100 mg/（kg·d））AEBLS后注射2 mL/kg CCl4诱导小鼠发生肝损伤，观察小鼠肝指数、血清指标和肝组织中相关mRNA表达的强度。结果显示，AEBLS可以降低肝损伤小鼠的肝质量和肝指数（P＜0.05）；AEBLS可以下调肝损伤小鼠血清中的天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶、甘油三酯、总胆固醇、尿素氮、一氧化氮、丙二醛水平和上调白蛋白、超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）水平（P＜0.05）；同时AEBLS还可以下调肝损伤小鼠血清中白介素-6（interleukin-6，IL?6）、IL?12、肿瘤坏死因子-α（tumor necrosis factor-alpha，TNF-α）和γ-干扰素细胞因子水平（P＜0.05）。实时聚合酶链式反应也证实AEBLS能上调肝损伤小鼠肝组织中Mn-SOD、Cu/Zn-SOD、CAT、GSH-Px、核因子κB抑制蛋白α的mRNA表达和下调核因子κB-p65、诱导型一氧化氮合成酶、环氧化酶-2、TNF-α、IL-1β、IL-6的mRNA表达（P＜0.05）。由此可见，AEBLS对CCl4引发的肝损伤有较好的预防作用，且效果接近药物水飞蓟素，具有较好的功能性作用。

关键词: 巴莲莲子, 生物碱, mRNA表达, 肝指数, 谷胱甘肽过氧化物酶

Abstract: The aim of this study was to determine the preventive effects of alkaloids extracted from Ba lotus seeds (AEBLS) on CCl4-induced hepatic injury in mice. AEBLS was purified using 732 exchange resin and then orally administered to mice. Afterwards, the mice were injected with CCl4 to induce hepatic injury, and liver index, serum biochemical parameters, and the mRNA expression of related genes in hepatic tissue were evaluated. The results showed that AEBLS could reduce liver weight and liver index in mice with hepatic injury. AEBLS could decrease aspartate aminotransferase (AST), aminotransferase (ALT), alkaline phosphatase (ALP), triglyceride (TG), total cholesterol (TC), urea nitrogen (BUN), nitric oxide (NO) and malondialdehyde (MDA) levels and increase albumin (ALB), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) levels of the serum of mice with hepatic injury. Meanwhile, it could also increase serum levels of cytokines such as interleukin-6 (IL?6), IL?12, tumor necrosis factor-alpha (TNF-α) and interferon-γ (IFN-γ). RT-PCR results also confirmed that AEBLS could upregulate Mn-SOD, Cu/Zn-SOD, CAT, GSH-Px and IκB-α mRNA expression and downregulate NF-κB-p65, iNOS, COX-2, TNF-α, IL-1β and IL-6 mRNA expression in the liver tissue of mice with hepatic injury. Therefore, AEBLS has a good preventive effect on CCl4-induced hepatic injury, which is close to that of silymarin.

Key words: Ba lotus seed, alkaloid, mRNA expression, liver index, glutathione peroxidase

中图分类号:

TS201.4

冯霞，孙鹏，易若琨，彭德光，赵欣. 巴莲莲子生物碱提取物对CCl4诱导小鼠肝损伤的预防效果[J]. 食品科学, 2017, 38(17): 216-222.

FENG Xia, SUN Peng, YI Ruokun, PENG Deguang, ZHAO Xin. Preventive Effects of Alkaloids Extracted from Ba Lotus Seeds on CCl4-Induced Hepatic Injury in Mice[J]. FOOD SCIENCE, 2017, 38(17): 216-222.

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巴莲莲子生物碱提取物对CCl4诱导小鼠肝损伤的预防效果

Preventive Effects of Alkaloids Extracted from Ba Lotus Seeds on CCl4-Induced Hepatic Injury in Mice

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