食品科学 ›› 2017, Vol. 38 ›› Issue (19): 149-155.doi: 10.7506/spkx1002-6630-201719024

• 基础研究 • 上一篇    下一篇

费菜黄酮对冷却猪肉腐败菌及品质的影响

王春幸,王鸿飞*,熊 茜,宋佳敏,董栓泉,许 凤,邵兴锋,李和生   

  1. 宁波大学食品科学与工程系,浙江 宁波 315211
  • 出版日期:2017-10-15 发布日期:2017-09-29
  • 基金资助:
    浙江省自然科学基金项目(LY16C200003);宁波市自然科学基金项目(2015A610273); 浙江省重点研发项目(2016C02G4010891)

Effect of Flavonoids from Sedum aizoon L. on Spoilage Bacteria and Quality of Chilled Pork

WANG Chunxing, WANG Hongfei*, XIONG Qian, SONG Jiamin, DONG Shuanquan, XU Feng, SHAO Xingfeng, LI Hesheng   

  1. Department of Food Science and Engineering, Ningbo University, Ningbo 315211, China
  • Online:2017-10-15 Published:2017-09-29

摘要: 通过二倍稀释法和牛津杯法确定了费菜黄酮对3 种冷却猪肉腐败菌的最低抑菌浓度(minimum inhibitory concentration,MIC)、最低杀菌浓度(minimum bactericidal concentration,MBC)和抑菌活性,并研究了不同质量 浓度费菜黄酮对猪肉的保鲜效果。结果表明,费菜黄酮对实验菌有不同程度的抑制作用,其中对于气单胞菌的抑制 性最强,MIC和MBC分别为0.675、2.5 mg/mL,假单胞菌次之,MIC和MBC分别为1.25、5 mg/mL,乳酸菌抑制性 最差,MIC和MBC分别为1.25、10 mg/mL。综合菌落总数和色差指标,选择2 mg/mL作为费菜黄酮对冷却猪肉保鲜 的适宜质量浓度。在4 ℃贮藏条件下,黄酮处理组组织结构、pH值、持水力、挥发性盐基氮值和硫代巴比妥酸值各 品质指标均优于对照组。

关键词: 费菜黄酮, 抑菌活性, 猪肉, 品质

Abstract: The antimicrobial activity, minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of flavonoids extracted from Sedum aizoon L. on three spoilage bacteria isolated from chilled meat were investigated by double-tube dilution method and the Oxford-cup method. The effect of the flavonoids at different concentrations on pork preservation was also examined. The results showed that the flavonoids displayed different inhibitory effects on the tested bacteria and had the strongest antimicrobial activity against Aeromonas with MIC and MBC 0.675 and 2.5 mg/mL, respectively, followed by against Pseudomonad with MIC and MBC of 1.25 and 5 mg/mL, and lactic acid bacteria with MIC and MBC of 1.25 and 10 mg/mL, respectively. Based on the total number of colonies and color, 2 mg/mL of the flavonoids was determined to be appropriate for the preservation of pork. The texture, pH, water-holding capacity, total volatile basic nitrogen (TVB-N) and 2-thiobarbituric acid (TBA) values in flavonoids-treated group were better than those in the control group during storage at 4 ℃.

Key words: Sedum aizoon L. flavonoid, antimicrobial activity, pork, quality

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