细胞水平评价沙棘粕醇提物的抗衰老功效

doi:10.7506/spkx1002-6630-201719026

食品科学 ›› 2017, Vol. 38 ›› Issue (19): 164-170.doi: 10.7506/spkx1002-6630-201719026

细胞水平评价沙棘粕醇提物的抗衰老功效

张佳婵1，史豆豆1，王昌涛1,2,*，李萌1，赵丹1，王成涛2,3，孙宝国2,3

1.北京工商大学理学院，植物资源研究开发北京市重点实验室，北京 100048；2.北京工商大学北京食品营养与人类健康高精尖创新中心，北京 100048；3.北京工商大学食品学院，食品添加剂与配料北京高校工程研究中心，北京 100048

出版日期:2017-10-15 发布日期:2017-09-29
基金资助:
国家自然科学基金面上项目（31571801）

Anti-Aging Effects of Sea Buckthorn Seed Extracts

ZHANG Jiachan1, SHI Doudou1, WANG Changtao1,2,*, LI Meng1, ZHAO Dan1, WANG Chengtao2,3, SUN Baoguo2,3

1. Beijing Key Laboratory of Plant Resources Research and Development, School of Science, Beijing Technology and Business University, Beijing 100048, China; 2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; 3. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China

Online:2017-10-15 Published:2017-09-29

摘要/Abstract

摘要： 本实验以沙棘粕为研究对象，提取得到沙棘粕醇提物（sea buckthorn seed extracts，SBSE），对SBSE进行极性部位划分，得到乙酸乙酯层和萃余水层；对划分部分及SBSE（下称“粗提物”）进行活性成分分析、化学水平自由基清除能力测定以及细胞水平过氧化氢酶活力、活性氧含量、基质金属蛋白酶1（matrix metalloproteinase-1， MMP-1）含量和Ⅰ型胶原含量分析。结果表明，粗提物、乙酸乙酯层和萃余水层主要活性成分均为黄酮类、原花青素类和总酚，三者均具有较为显著的1,1-二苯基-2-三硝基苯肼自由基清除能力，乙酸乙酯层和萃余水层对羟自由基（·OH）的清除能力显著，粗提物未表现出对·OH的清除效果；3 种样品在0.05～5.00 mg/mL 范围内，对人皮肤成纤维细胞均未表现出强烈的细胞毒性，细胞活力均在80%以上；3 种样品刺激细胞后，其过氧化氢酶活力显著升高，除0.5 mg/mL萃余水层外，其余样品作用后细胞中活性氧水平高度显著降低（P＜0.001），其中，粗提物比乙酸乙酯层和萃余水层具有更为优越的促进过氧化氢酶活性和抑制活性氧产生的能力；并且，3 种样品特别是粗提物可以显著降低人皮肤成纤维细胞中MMP-1的含量（P＜0.05），其细胞中Ⅰ型胶原含量也最高。由结果可知，SBSE具有潜在的抗衰老功效。

关键词: 沙棘粕, 粗提物, 乙酸乙酯层, 萃余水层, 抗衰老

Abstract: In this research, sea buckthorn seed extracts (SBSE) were obtained by the extraction of sea buckthorn seed residues using ethanol. SBSE were separated into two fractions of different polarities: the upper ethyl acetate phase and the bottom aqueous phase. The crude extract and its fractions were tested for bioactive components, chemical and cellular antioxidant properties including scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radicals and catalase activity, and their effects on reactive oxygen (ROS), matrix metalloproteinase-1 (MMP-1) and type Ⅰ collagen (COLⅠ) contents in the culture supernatants of human skin fibroblast cells were examined. The results showed that flavonoids, proanthocyanidins and total phenolics were the major bioactive compounds in the crude extract and its fractions. All these samples could strongly scavenge DPPH free radical. The two fractions but not the crude extract showed potent hydroxyl radical scavenging activity. All three samples in the concentration range of 0.05–5.00 mg/mL showed no strong cytotoxicity to human skin fibroblast cells and the cell viability was above 80% in the presence of each sample. Stimulation with the samples significantly increased catalase activity and reduced ROS content, and the crude extract showed the strongest catalase activity and lowest ROS level among these samples. Furthermore, cellular MMP-1 contents were significantly decreased by SBSE, and COLⅠ contents were increased to the maximum. Therefore, SBSE had potential anti-aging effect.

Key words: sea buckthorn seed residues, crude extracts, ethyl acetate phase, aqueous phase, anti-aging potential

中图分类号:

TS209

张佳婵，史豆豆，王昌涛，李萌，赵丹，王成涛，孙宝国. 细胞水平评价沙棘粕醇提物的抗衰老功效[J]. 食品科学, 2017, 38(19): 164-170.

ZHANG Jiachan, SHI Doudou, WANG Changtao, LI Meng, ZHAO Dan, WANG Chengtao, SUN Baoguo. Anti-Aging Effects of Sea Buckthorn Seed Extracts[J]. FOOD SCIENCE, 2017, 38(19): 164-170.

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细胞水平评价沙棘粕醇提物的抗衰老功效

Anti-Aging Effects of Sea Buckthorn Seed Extracts

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