食品科学 ›› 2017, Vol. 38 ›› Issue (20): 154-160.doi: 10.7506/spkx1002-6630-201720022

• 工艺技术 • 上一篇    下一篇

沙棘渣制备微晶纤维素的酶解条件优化

盛文军,毕阳,冯丽丹,李霁昕,韩舜愈,李敏   

  1. (甘肃农业大学食品科学与工程学院,甘肃?兰州 730070)
  • 出版日期:2017-10-25 发布日期:2017-09-29
  • 基金资助:
    国际科技合作重点计划项目(2014DFR31230)

Optimization of Enzymatic Hydrolysis Conditions for Preparation of Microcrystalline Cellulose from Seabuckthorn Pomace

SHENG Wenjun, BI Yang, FENG Lidan, LI Jixin, HAN Shunyu, LI Min   

  1. (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)
  • Online:2017-10-25 Published:2017-09-29

摘要: 沙棘浆加工过程中产生酚酸含量高的果渣,因其苦涩无法被饲料工业大量转化,利用其进行微晶纤维素制备是潜在可行的解决途径。以粗提沙棘渣纤维素为处理对象,使用S10041纤维素酶水解,选取液料比、酶添加量、酶解时间、酶解温度、缓冲液pH值、离心转速、烘干温度及纤维素粉碎度8?个因素,通过单因素试验和Plackett-Burman因素筛选,并经过最陡爬坡试验和Box-Behnken试验优化了酶解条件,随后对制得的微晶纤维进行结构分析。结果表明:在液料比49∶1(mL/g)、酶添加量68?U/mL、酶解时间1.3?h、离心转速3?640?r/min时制得的沙棘微晶纤维素聚合度为355±1.02,与棉微晶纤维素聚合度最为接近。方差分析表明4?个选定因素对指标均产生独立影响,因素交互作用对指标影响不显著(P=0.10)。微观结构显示沙棘微晶纤维表面结构更疏松,红外图谱对比沙棘和棉花两种微晶纤维官能团结构相似。

关键词: 沙棘渣, 微晶纤维素, 纤维素酶

Abstract: Seabuckthorn pomace is rich in phenolic acid and is a byproduct produced during the processing of seabuckthorn pulp. However, the astringency and bitterness of seabuckthorn pomace hinder its utilization in the feed industry. The preparation of microcrystalline cellulose from seabuckthorn pomace is a potential solution to this problem. Therefore, in this study, the crude cellulose extracted from seabuckthorn pomace was hydrolyzed with commercial cellulase S10041 to obtain microcrystalline cellulose. Eight processing parameters were investigated, namely solid-to-solvent ratio, enzyme dosage, hydrolysis time, temperature, buffer pH, centrifugal rotational speed, drying temperature, and comminution degree of cellulose. The significant factors were selected and optimized using one-factor-at-a-time method, Plackett-Burman design, steepest ascent path design and Box-Behnken design combined with response surface methodology. The prepared microcrystalline cellulose was structurally elucidated. The results showed that the degree of polymerization of seabuckthorn microcrystalline cellulose was 355 ± 1.02 under the optimal follows: ratio of buffer solution to cellulose, 49:1 (mL/g); enzyme dosage, 68 U/mL; hydrolysis time, 1.3 h; and centrifugal rotational speed, 3 640 r/min, which was close to that of cotton microcrystalline cellulose. The analysis of variance showed that the four selected factors had independent influences on the degree of polymerization but their interactions had no significant effect on the response (P = 0.10). The scanning electron micrograph revealed that the surface structure of seabuckthorn microcrystalline cellulose was more porous as compared to cotton microcrystalline cellulose. The infrared spectra of two microcrystalline celluloses revealed that similar functional groups existed.

Key words: seabuckthorn pomace, microcrystalline cellulose, cellulase

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