食品科学 ›› 2017, Vol. 38 ›› Issue (20): 185-190.doi: 10.7506/spkx1002-6630-201720026

• 工艺技术 • 上一篇    下一篇

花脸香蘑菌丝体多糖提取条件优化及其体外免疫活性

胡欣蕾,田雪梅,李文香,孙亚男,张欣,于戈,李铭   

  1. (1.青岛农业大学食品科学与工程学院,山东省应用真菌重点实验室,山东?青岛 266109; 2.青岛农业大学生命科学学院,山东?青岛 266109)
  • 出版日期:2017-10-25 发布日期:2017-09-29
  • 基金资助:
    山东省科技发展计划项目(2014GNC113007);山东省现代农业产业技术体系建设经费资助项目(SDAIT-07-07)

Extraction and Immune Modulatory Activities in Vitro of Polysaccharides from Lepista sordida Mycelia

HU Xinlei, TIAN Xuemei, LI Wenxiang, SUN Yanan, ZHANG Xin, YU Ge, LI Ming   

  1. (1. Shandong Provincial Laboratory of Applied Mycology, College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China; 2. College of Life Science, Qingdao Agricultural University, Qingdao 266109, China)
  • Online:2017-10-25 Published:2017-09-29

摘要: 为探讨花脸香蘑菌丝体多糖提取工艺及其免疫活性,以花脸香蘑菌丝体为原料,在单因素试验的基础上采用正交试验优化多糖提取条件;将不同质量浓度的花脸香蘑菌丝体多糖溶液作用于小鼠巨噬细胞Ana-1,通过对其细胞存活率、细胞因子TNF-α和IL-6分泌量、中性红吞噬能力以及膜表面蛋白Toll样受体(Toll-like receptor,TLR)TLR2和TLR4表达量能力的测定,研究花脸香蘑菌丝体多糖的免疫活性。结果表明,花脸香蘑菌丝体多糖最佳提取条件为浸提温度65?℃、浸提时间2.5?h、液料比40∶1(mL/g),提取率最高为15.88%;在0.05~1.6?mg/mL的有效剂量范围内,花脸香蘑菌丝体多糖能够不同程度地促进小鼠巨噬细胞Ana-1的增殖,提高细胞因子TNF-α和IL-6分泌量,增强中性红吞噬能力、膜表面蛋白TLR2和TLR4表达量能力,其中以1.6?mg/mL效果最佳。表明花脸香蘑菌丝体多糖具有优良的免疫活性。

关键词: 花脸香蘑菌丝体, 多糖, 提取工艺, 正交试验, 免疫活性

Abstract: This study aimed to improve the extraction yield and evaluate the immune modulatory activity of polysaccharides of Lepista sordida mycelia. Optimization of the extraction conditions was carried out using one-factor-at-a-time and orthogonal array design methods. The immune modulatory effect of the polysaccharides extracted was evaluated by measuring cell survival, TNF-α and IL-6 secretion, the percentage of neutral red phagocytosis and the expression levels of Toll-like receptor (TLR) TLR2 and TLR4 when mouse Ana-1 macrophages were exposed to different concentrations of the polysaccharides. The optimal extraction conditions were determined as follows: extraction temperature, 65 ℃; extraction time, 2.5 h; solvent-to-solid ratio, 40:1 (mL/g), leading to an extraction yield of 15.88%. In the concentration range of 0.05–1.6 mg/mL, the polysaccharides could promote the proliferation of macrophages, TLR2 and TLR4 expression, the phagocytosis of neutral red and TNF-α and IL-6 production; the most effective concentration was 1.6 mg/mL. These data illustrated that the polysaccharides possessed good immune modulatory activity.

Key words: Lepista sordida mycelium, polysaccharides, extraction, orthogonal array design, immune modulatory activities

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