食品科学 ›› 2017, Vol. 38 ›› Issue (22): 28-33.doi: 10.7506/spkx1002-6630-201722005

• 生物工程 • 上一篇    下一篇

烟台地区引起甜樱桃采后腐烂霉菌的分离鉴定

田亚晨,贡汉生,赵珊,刘文丽,南树港,杨婷   

  1. (鲁东大学食品工程学院,山东?烟台 264025)
  • 出版日期:2017-11-25 发布日期:2017-11-03
  • 基金资助:
    国家自然科学基金青年科学基金项目(31301565);山东省自然科学基金青年基金项目(ZR2012CQ009); 烟台市科技计划项目(2012125);山东省高等学校科技计划项目(J12LD03)

Screening and Identification of Spoilage Moulds from Postharvest Sweet Cherry in Yantai

TIAN Yachen, GONG Hansheng, ZHAO Shan, LIU Wenli, NAN Shugang, YANG Ting   

  1. (School of Food Engineering, Ludong University, Yantai 264025, China)
  • Online:2017-11-25 Published:2017-11-03

摘要: 为明确引起烟台地区甜樱桃采后腐烂的主要霉菌,对烟台采后腐烂甜樱桃中的霉菌进行分离,应用内部转录间隔区序列分析方法和形态学观察将分离的霉菌进行鉴定。选出代表性霉菌菌株回接樱桃,进行致腐性检测。结果表明:从烟台不同区县收集的腐烂樱桃中分离出的60?株霉菌分属于6?个种:燕麦赤霉菌(Gibberella avenacea)、总状毛霉菌(Mucor racemosus)、三线镰刀菌(Fusarium tricinctum)、互生链格孢菌(Alternaria alternate)、奥桑青霉菌(Penicillium polonicum)和烟曲霉菌(Aspergillus fumigatus),其中互生链格孢菌和总状毛霉数量较多,分别为14 株和15 株。将这些霉菌中的代表菌株重新接回到甜樱桃,均能引起甜樱桃的腐烂。

关键词: 甜樱桃, 霉菌, 分离鉴定

Abstract: In order to clarify the dominant mould species causing postharvest decay of sweet cherry in Yantai, spoilage moulds from decayed sweet cherry fruits were screened and identified by internal transcribed spacer (ITS) sequence analysis. Typical strains were examined for colonial morphology and then re-inoculated into sweet cherry to test their pathogenicity. A total of 60 moulds were isolated from decayed sweet cherry from different regions of Yantai, and they were assigned to 6 species based on both ITS sequence analysis and morphological identification, Mucor racemosu, Fusarium tricinctum, Alternaria alternate, Penicillium polonicum and Aspergillus fumigatus. The predominant species were Alternaria alternate (14 strains) and Mucor racemosu (15 strains). Compared with naturally decayed sweet cherry, sweet cherries inoculated with moulds were decayed and displayed the same colonial morphology.

Key words: sweet cherries, moulds, screening and identification

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