食品科学 ›› 2017, Vol. 38 ›› Issue (23): 14-19.doi: 10.7506/spkx1002-6630-201723003

• 基础研究 • 上一篇    下一篇

米糠水溶性膳食纤维理化特性及抗氧化性

黄 萍,林亲录,朱凤霞,王玉倩,崔校基,梁 盈*   

  1. 中南林业科技大学 稻谷及副产物深加工国家工程实验室,湖南 长沙 410004
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    国家自然科学基金青年科学基金项目(31201348);国家自然科学基金面上项目(31571874); 湖南省自然科学基金面上项目(2017JJ2410);2016湖湘青年科技创新人才项目(2016RS3033); 粮油深加工与品质控制2011湖南省协同创新项目;中南林业科技大学研究生教材建设项目(2015JC03)

Physicochemical Properties and Antioxidant Activity of Water-Soluble Dietary Fiber from Rice Bran

HUANG Ping, LIN Qinlu, ZHU Fengxia, WANG Yuqian, CUI Xiaoji, LIANG Ying*   

  1. National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Changsha 410004, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 以超声辅助纤维素酶水解脱脂米糠提取得到的水溶性膳食纤维(water-soluble dietary fibre,SDF)为原 料,研究其理化性质和功能特性,发现米糠SDF纯度较高,为(79.52±0.07)%,其蛋白质和灰分含量少,分别为 (7.82±0.13)%、(5.71±0.11)%。优化提取的米糠SDF具有良好的理化性质:持水力为(3.16±0.15)g/g、持 油力为(0.99±0.08)g/g、溶胀性为(1.39±0.12)mL/g、结合水力为(4.18±0.10)g/g。米糠SDF具有一定的阳离 子交换能力,其溶解性与温度成正比。傅里叶变换红外光谱分析发现,米糠SDF中处于缔合状态的氢键较多;扫描 电子显微镜分析超微结构结果表明其纯度较高。米糠SDF表现出较强的抗氧化能力,随质量浓度的升高呈明显上升 趋势,10 mg/mL的SDF对1,1-二苯基-2-三硝基苯肼自由基清除率为20.27%,对2’-联氨-双-3-乙基苯并噻唑啉-6-磺酸 自由基清除率达到58.97%。

关键词: 米糠, 水溶性膳食纤维, 理化特性, 抗氧化活性

Abstract: This study evaluated the physicochemical and functional properties of water-soluble dietary fiber (SDF) prepared by ultrasonic-assisted cellulase hydrolysis of defatted rice bran. The SDF, with a high purity of (79.52 ± 0.07)%, had low protein and ash contents of (7.82 ± 0.13)% and (5.71 ± 0.11)%, respectively. The SDF had good physical and chemical properties; its water-holding capacity was (3.16 ± 0.15) g/g, oil-holding capacity (0.99 ± 0.08) g/g, swelling capacity (1.39 ± 0.12) mL/g, and water-binding capacity (4.18 ± 0.10) g/g. It was also found that the SDF had cation exchange capacity, and its solubility was positively proportional to temperature. Fourier transform infrared spectral analysis indicated the presence of some associated hydrogen bond in the SDF. Scanning electron microscopic analysis confirmed its high purity. Furthermore, the SDF exhibited strong antioxidant capacity in a concentration-dependent manner, which was determined to be 20.27% and 58.97% in the scavenging of 1,1-diphenyl-2-picrylhydrazyl and 2,2’-azinobis-(3-ethylbenzthiazoline-6- sulphonate) radicals at 10 mg/mL, respectively.

Key words: rice bran, water-soluble dietary fiber, physicochemical properties, antioxidant activity

中图分类号: