食品科学 ›› 2017, Vol. 38 ›› Issue (23): 149-156.doi: 10.7506/spkx1002-6630-201723024

• 营养卫生 • 上一篇    下一篇

香蕉抗性淀粉对C57BL/6J肥胖小鼠肠道放线菌群的调控作用

李汉荣1,黄淑君1,2,曾本华3,方 祥1,王 丽1,钟青萍1,童贻刚4,魏 泓3,*,廖振林1,*   

  1. 1.华南农业大学食品学院,广东 广州 510642;2.广东省妇幼保健院,广东 广州 511400;3.第三军医大学基础部实验动物学教研室,重庆 400038;4.军事医学科学院微生物流行病学研究所,北京 100071
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    国家自然科学基金面上项目(31171673;31071528;31671855;81370906); 国家重点实验室开放课题(SKLPBS1518);国家重点基础研究发展计划(973)项目(2013CB532406); 国家高技术研究发展计划(863)项目(2014AA022209;2014AA022204)

Regulative Effect of Dietary Banana Resistant Starch on Gut Actinobacteria in Obese C57BL/6J Mice

LI Hanrong1, HUANG Shujun1,2, ZENG Benhua3, FANG Xiang1, WANG Li1, ZHONG Qingping1, TONG Yigang4, WEI Hong3,*, LIAO Zhenlin1,*   

  1. 1. College of Food Science, South China Agricultural University, Guangzhou 510642, China;2. Guangdong Maternal and Child Health Hospital, Guangzhou 511400, China; 3. Department of Laboratory Animal Science, College of Basic Medical Sciences, the Third Military Medical University, Chongqing 400038, China; 4. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 目的:研究不同剂量香蕉抗性淀粉(resistant starch,RS)对高脂饮食诱导的C57BL/6J肥胖小鼠肠道放线 菌群多样性的影响。方法:将40 只C57BL/6J小鼠随机分为5 组,分别采用普通饲料(CONV)、高脂饲料(HF) 以及添加5%、10%、15%香蕉抗性淀粉的高脂饲料(5% RS+HF、10% RS+HF、15% RS+HF)进行饮食干预, 8 周后采用变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术对小鼠粪便样本放线菌菌群组 成进行分析,采用实时荧光定量聚合酶链式反应技术对小鼠粪便样本双歧杆菌数量进行比较。结果:DGGE图谱分 析显示,HF组聚类与其余4 组彻底分离,抗性淀粉各组趋于成簇但无明显界限。5% RS+HF组与15% RS+HF组放 线菌多样性和丰富度方面均显著低于CONV组与HF组(P<0.05),10% RS+HF组多样性和丰富度较另外2 组RS+ HF组有所上升。高脂饲料能够极显著降低肠道中双歧杆菌数量(P<0.01),而10%和15%香蕉抗性淀粉的加入均 高度显著增加双歧杆菌数量(P<0.001)。结论:香蕉抗性淀粉可恢复肥胖小鼠肠道放线菌群多样性,显著促进双 歧杆菌生长,且具有剂量依赖关系。

关键词: 香蕉抗性淀粉, 肠道放线菌, 变性梯度凝胶电泳

Abstract: To study the impact of a high-fat diet supplemented with different doses of banana resistant starch (RS) on the diversity of gut Actinobacteria in obese C57BL/6J mice, 40 mice were randomly divided into five groups, which were fed a conventional diet (CONV), a high-fat diet (HF) alone, supplemented with 5% (5% RS + HF), 10% (10% RS + HF), and 15% (15% RS + HF) of RS, respectively. After 8 weeks of dietary intervention, denaturing gradient gel electrophoresis (DGGE) was used to monitor the changes in the composition of gut Actinobacteria in mice. The amounts of fecal Bifidobacterium were compared among the five groups of animals by real-time fluorescence quantitative polymerase chain reaction (PCR). Cluster analysis of the DGGE profiles showed that the HF group was significantly different from the other 4 groups. All the RS groups tended to form a cluster without obvious boundaries. In addition, the diversity and abundance of Actinobacteria in the 5% RS + HF and 15% RS + HF groups were significantly lower than in the CONV and HF groups (P < 0.05) and also lower than in the 10% RS + HF group. PCR results suggested that the high-fat diet could highly significantly reduce the number of gut Bifidobacteria (P < 0.01), which, however, was very significantly increased by supplementation of 10% and 15% RS (P < 0.001). Hence, we concluded that resistant starch can restore the diversity of gut Actinobacteria in obese mice, and significantly promote the growth of Bifidobacteria in a dose-dependent manner.

Key words: banana resistant starch, gut Actinobacteria, denaturing gradient gel electrophoresis (DGGE)

中图分类号: