食品科学 ›› 2017, Vol. 38 ›› Issue (23): 269-274.doi: 10.7506/spkx1002-6630-201723043

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超高压处理对三疣梭子蟹感官及其肌原纤维蛋白生化特性的影响

周 果1,2,杨文鸽2,崔 燕1,王芝妍2,俞静芬1,林旭东1,凌建刚1,*   

  1. 1.宁波市农业科学研究院,宁波市农产品保鲜工程重点实验室,浙江 宁波 315040;2.宁波大学海洋学院,浙江省动物蛋白食品精深加工重点实验室,浙江 宁波 315211
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    宁波市重大农业科技攻关项目(2014C10009);宁波市公益类重大科技专项(2015C110002)

Effect of Ultra-High Pressure on Sensory and Biochemical Properties of Myofibrillar Protein from Portunus trituberculatus

ZHOU Guo1,2, YANG Wenge2, CUI Yan1, WANG Zhiyan2, YU Jingfen1, LIN Xudong1, LING Jiangang1,*   

  1. 1. Key Laboratory of Preservation of Agricultural Products of Ningbo, Ningbo Academy of Agriculture Sciences, Ningbo 315040, China; 2. Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province, School of Marine Science, Ningbo University, Ningbo 315211, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 以不同的压力处理三疣梭子蟹,通过考察其感官、pH值、色泽、肌原纤维蛋白含量、巯基含量、表面疏 水性和Ca2+-ATPase活力的变化,研究超高压处理对梭子蟹品质的影响。结果表明,150、200 MPa以及250 MPa 处理后的梭子蟹,其感官评分、pH值和白度无显著变化(P>0.05),而300 MPa和350 MPa组均出现显著性变化 (P<0.05),且蟹肉出现熟化,产生了微弱的熟蟹肉特有风味;与对照组相比,250 MPa组的肌原纤维蛋白含量和 150、200 MPa组的Ca2+-ATPase活力变化不显著(P>0.05),其余处理组均发生显著性变化(P<0.05);150 MPa 和200 MPa组蟹肉肌原纤维蛋白的巯基含量没有显著性变化(P>0.05);而表面疏水性与对照组相比均显著升高 (P<0.05)。综合梭子蟹感官评分及其肌原纤维蛋白生化特性指标,150、200 MPa和250 MPa的压力不会引起梭 子蟹品质显著改变,这为后续研究超高压对梭子蟹贮藏品质的影响提供了理论依据。

关键词: 超高压, 三疣梭子蟹, 感官评价, 肌原纤维蛋白, 生化特性

Abstract: In order to study the effect of ultra-high pressure treatment on the quality of freshly dead Portunus trituberculatus, changes in sensory scores, pH, color difference, myofibrillar protein content, sulphydryl content, surface hydrophobicity and Ca2+-ATPase activity were determined after high pressure treatments. Results showed that sensory scores, pH and whiteness of P. trituberculatus did not significantly change at 150, 200 and 250 MPa compared with the control group (P > 0.05), but significant changes occurred at 300 and 350 MPa (P < 0.05). Moreover, at the higher pressures crab muscle was slightly cooked, producing a unique flavor of cooked crab. At all pressures except 250 MPa for myofibrillar protein content and except 150 and 200 MPa for Ca2+-ATPase activity, both parameters significantly changed (P < 0.05). No significant change was observed in sulfhydryl content of crab myofibrillar protein at 150 and 200 MPa (P > 0.05), whereas surface hydrophobicity was significantly increased (P < 0.05) compared with the control group. Taking into account both sensory scores and biochemistry properties of myofibrillar protein, the quality of P. trituberculatus didn’t significantly changed at 150, 200 or 250 MPa, which can provide a theoretical basis for further studying the effect under ultra-high pressure on the storage quality of P. trituberculatus.

Key words: ultra-high pressure, Portunus trituberculatus, sensory evaluation, myofibrillar protein, biochemical properties

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