食品科学 ›› 2019, Vol. 40 ›› Issue (5): 214-221.doi: 10.7506/spkx1002-6630-20180112-156

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采后苯并噻重氮处理对‘玉金香’甜瓜单萜类香气及其代谢关键酶的影响分析

王 雨1,李霁昕1,李经纬2,郝兰兰1,胡妍芸1,王 博1,蒋玉梅1,*   

  1. 1.甘肃农业大学食品科学与工程学院,甘肃省葡萄与葡萄酒工程学重点实验室,甘肃省葡萄酒产业技术研发中心,甘肃 兰州 730070;2.甘肃出入境检验检疫局综合技术中心,甘肃 兰州 730070
  • 出版日期:2019-03-15 发布日期:2019-04-02
  • 基金资助:
    国家自然科学基金地区科学基金项目(31160344)

Effect of Postharvest BTH Treatment on Aroma Monoterpenes and Key Metabolic Enzymes in ‘Yujinxiang’ Melon

WANG Yu1, LI Jixin1, LI Jingwei2, HAO Lanlan1, HU Yanyun1, WANG Bo1, JIANG Yumei1,*   

  1. 1. Gansu Key Laboratory of Viticulture and Enology, Research and Development Center of Wine Industry of Gansu Province, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; 2. Comprehensive Technical Center of Gansu Entry-Exit Inspection and Quarantine Bureau, Lanzhou 730070, China
  • Online:2019-03-15 Published:2019-04-02

摘要: 以‘玉金香’甜瓜(Cucumis melo L.)为实验材料,监测采后常温贮藏期间单萜类香气组分代谢l-脱氧-D-木酮糖-5-磷酸还原异构酶(l-deoxy-D-xylulose-5-phosphate reductoisomerase,DXR)活力及其单萜类香气组分的变化,探讨采后苯并噻重氮(benzo-(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester,BTH)诱抗处理对DXR活力和单萜类香气构成的影响,分析DXR活力与单萜类香气物质释放量的相关性。结果表明:贮藏期间,样品中共检出9 种单萜类香气组分,其中8 种单萜类香气物质含量与DXR活力呈正相关;采后BTH处理抑制了甜瓜DXR活力和单萜类香气物质的释放,延迟了DXR活力峰值出现,改变了单萜类香气释放规律及其释放量与DXR活力的相关性。与CC组(蒸馏水浸泡)相比,BTH处理组果皮、果肉样DXR活力分别降低了19.48%、25.49%;条件因子水可增大DXR活力峰值,但对DXR活力和单萜类香气的影响无明显规律。由此可见,采后BTH处理可通过抑制‘玉金香’甜瓜DXR活力,延迟活性峰值出现,改变单萜类香气释放量与DXR活力的相关性,影响单萜类香气的释放。

关键词: 苯丙噻重氮, l-脱氧-D-木酮糖-5-磷酸还原异构酶, 单萜类香气物质

Abstract: The objective of this study was to investigate the effect of postharvest treatment with the plant resistance inducer benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) on the activity of l-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) and aroma-active monoterpenes in muskmelon (Cucumis melo L. cv. Yujinxiang) and to analyze the correlation between DXR activity and the release of aroma-active monoterpenes. Changes in DXR activity, involved in the metabolic pathway of aroma-active monoterpenes and monoterpene composition were monitored during postharvest storage at room temperature. The results showed that a total of nine aroma-active monoterpene components were detected in the samples during the storage period. Eight of these compounds were positively correlated with DXR activity. Upon postharvest BTH treatment, DXR activity and the release of monoterpenes were inhibited accompanied by delayed peak time of DXR activity as well as altered release pattern of aroma-active monoterpenes and correlation with DXR activity. Compared with the CC group (distilled water immersion), DXR activity in the rind and pulp of BTH-treated melon was reduced by 19.48% and 25.49%, respectively. The effect of distilled water immersion on DXR activity and the release of aroma-active monoterpenes was irregular although it increased the peak of DXR activity. From these findings, it is clear that postharvest BTH treatment can inhibit DXR activity, delay the peak time of DXR activity and affect the release of aromaactive monoterpenes and its correlation with DXR activity.

Key words: benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester, l-deoxy-D-xylulose-5-phosphate reductoisomerase, aroma-active monoterpenes

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