食品科学 ›› 2019, Vol. 40 ›› Issue (4): 192-198.doi: 10.7506/spkx1002-6630-20180120-276

• 成分分析 • 上一篇    下一篇

基于气相色谱-质谱技术与多元统计分析对不同栗香特征绿茶判别分析

尹洪旭1,2,杨艳芹1,姚月凤1,2,张铭铭1,2,王家勤1,2,江用文1,袁海波1,*   

  1. (1.中国农业科学院茶叶研究所?浙江省茶叶加工工程重点实验室,浙江?杭州 310008;2.中国农业科学院研究生院,北京 100081)
  • 出版日期:2019-02-25 发布日期:2019-03-05
  • 基金资助:
    国家自然科学基金面上项目(31471651);浙江省自然科学基金项目(LQ18C160006); 中国农业科学院创新工程项目(CAAS-ASTIP-2014-TRICAAS)

Discrimination of Different Characteristics of Chestnut-like Green Tea Based on Gas Chromatography-Mass Spectrometry and Multivariate Statistical Data Analysis

YIN Hongxu1,2, YANG Yanqin1, YAO Yuefeng1,2, ZHANG Mingming1,2, WANG Jiaqin1,2, JIANG Yongwen1, YUAN Haibo1,*   

  1. (1. Key Laboratory of Tea Processing Engineer of Zhejiang Province, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; 2. Graduate School of Chinese Academy of Agriculture Sciences, Beijing 100081, China)
  • Online:2019-02-25 Published:2019-03-05

摘要: 采用气相色谱-质谱联用技术结合多元统计分析主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least squares-discriminant analysis,PLS-DA)和系统聚类分析(hierarchical cluster analysis,HCA)对18?个不同栗香特征的绿茶开展研究。结果表明,PCA、PLS-DA和HCA均可直观地对3?种不同栗香特征的绿茶进行有效区分;PLS-DA中,18?个栗香茶样基于其香气特征实现良好分离,其中R2Y=0.843、Q2=0.694,说明该模型对3?种栗香特征绿茶具有良好的稳定性和较好的预测能力;HCA中,3种栗香绿茶在聚类距离12处被清晰地分成3?类,其中板栗香型和嫩栗香型距离更接近,聚类效果和感官辨识基本一致。此外,基于变量投影重要性大于1,筛选出了38?种区分不同栗香特征的重要挥发性组分。

关键词: 气相色谱-质谱, 主成分分析, 层次聚类分析, 偏最小二乘判别分析

Abstract: The volatile constitutions of 18 green tea samples with three different types of characteristic chestnut-like aroma were characterized based on gas chromatography-mass spectrometry (GC-MS) combined with multivariate statistical data analysis including principal component analysis (PCA), hierarchical cluster analysis (HCA) and partial least squares-discriminant analysis (PLS-DA). The results showed that PCA, PLS-DA and HCA could achieve good differentiation of three chestnut flavored green teas. In the PLS-DA analysis, 18 chestnut-like green tea samples were well separated according to their aroma characteristics, and the well-explained variance (R2Y =0.843) and cross-validated predictive capability (Q2 = 0.694) indicated the model’s good feasibility. In the HCA analysis, three kinds of chestnut fragrant green tea could be clearly divided into three categories at a distance of 12, of which the chestnut-like and tender chestnut-like tea samples were closer, matching the results of sensory evaluation. In addition, 38 volatile components were identified based on variable importance in projection (VIP) score > 1, which were responsible for the discrimination of green teas with three different flavor characteristics.

Key words: gas chromatography-mass spectrometry (GC-MS), principal component analysis (PCA), hierarchical cluster analysis (HCA), partial least squares-discriminant analysis (PLS-DA)

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