罗非鱼鱼鳞柠檬酸-苹果酸钙的制备及产物鉴定

doi:10.7506/spkx1002-6630-20180202-026

食品科学 ›› 2019, Vol. 40 ›› Issue (10): 265-271.doi: 10.7506/spkx1002-6630-20180202-026

罗非鱼鱼鳞柠檬酸-苹果酸钙的制备及产物鉴定

张玲1,2，谭南峰2，李春海1,2,*，周惠敏2，张钟1,2，李雯2

1.广东省岭南特色果蔬加工及应用工程技术研究中心，广东普通高校食品科学创新团队，广东茂名 525000；2.广东石油化工学院生物与食品工程学院，广东茂名 525000

出版日期:2019-05-25 发布日期:2019-05-31
基金资助:
广东省科技计划项目（2015B020230001）；广东普通高校食品科学创新团队项目（2016KCXTD020）；广东石油化工学院2017年大学生创新创业训练计划项目（201711656004）

Preparation and Identification of Calcium Citrate Malate from Tilapia Scale

ZHANG Ling1,2, TAN Nanfeng2, LI Chunhai1,2,*, ZHOU Huimin2, ZHANG Zhong1,2, LI Wen2

1. Technology Research Center for Lingnan Characteristic Fruits & Vegetables Processing and Application Engineering of Guangdong Province, Food Science Innovation Team of Guangdong Higher Education Institutes, Maoming 525000, China; 2. College of Biology and Food Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China

Online:2019-05-25 Published:2019-05-31

摘要/Abstract

摘要： 以罗非鱼鱼鳞为原料，采用超声辅助复合有机酸制备柠檬酸-苹果酸钙（calcium citrate malate，CCM），研究鱼鳞预处理方法、超声辅助酸制备工艺条件，并对产物进行表征。结果显示，鱼鳞无需熬煮，直接采用超声辅助提取效果理想；在液料比为20∶1（mL/g）条件下，正交试验优化得到复合钙最佳制备工艺条件为：柠檬酸和苹果酸总质量分数12%、二者质量比2∶1、超声温度70 ℃、超声时间1.0 h；在此条件下，得钙率为18.97%；红外光谱及X射线衍射分析均显示制备产物不是柠檬酸钙与苹果酸钙的简单混合物，而与CCM结构吻合；扫描电镜观察到产物结晶呈六方形；晶体比表面积介孔测定知罗非鱼鱼鳞复合钙产物孔径为4.049 nm，单点吸附总孔体积为0.320 cm3/g，T-图法微孔（外）比表面积为385.703 m2/g。本方法成功制备了罗非鱼鱼鳞CCM复合盐，得钙率较高，研究结果可为罗非鱼鱼鳞加工有机钙产品提供一定理论支持。

关键词: 罗非鱼鱼鳞, 柠檬酸-苹果酸钙, 超声波, 提取工艺, 结构表征

Abstract: In this paper, calcium citrate malate (CCM) was prepared by ultrasonic-assisted organic acid treatment of tilapia scale. The pretreatment and preparation conditions were optimized and the structure of the product were characterized. The results showed that good extraction efficiency was obtained by ultrasonic-assisted preparation of fish scale without boiling. At a solvent-to-solid ratio of 20:1 (mL/g), the optimum conditions for preparing CCM were obtained by orthogonal array design as follows: 12% (m/m) of an citric acid-malic acid mixture at a mass ratio of 2:1, temperature 70 ℃ and time 1.0 h. Under these conditions, the maximum extraction rate of calcium of 18.97% was obtained. Infrared spectroscopy analysis and X-ray diffraction (XRD) showed that the product was not a physical mixture of calcium citrate and calcium malate, but coincided with the structure of CCM. Scanning electron microscope showed that the crystal structure was a hexahedron. The specific surface area from mesoporous pore volume determination of the product was 385.703 m2/g, average pore size 4.049 nm and single point adsorption total pore volume 0.320 cm3/g. CCM was successfully prepared by this method with higher yield of calcium. These research results provide some theoretical support for the processing of organic calcium products from tilapia scale.

Key words: tilapia scale, calcium citrate malate (CCM), ultrasound, extraction process, structure characterization

中图分类号:

TS254.9

张玲，谭南峰，李春海，周惠敏，张钟，李雯. 罗非鱼鱼鳞柠檬酸-苹果酸钙的制备及产物鉴定[J]. 食品科学, 2019, 40(10): 265-271.

ZHANG Ling, TAN Nanfeng, LI Chunhai, ZHOU Huimin, ZHANG Zhong, LI Wen. Preparation and Identification of Calcium Citrate Malate from Tilapia Scale[J]. FOOD SCIENCE, 2019, 40(10): 265-271.

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罗非鱼鱼鳞柠檬酸-苹果酸钙的制备及产物鉴定

Preparation and Identification of Calcium Citrate Malate from Tilapia Scale

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