食品科学 ›› 2019, Vol. 40 ›› Issue (8): 112-118.doi: 10.7506/spkx1002-6630-20180212-146

• 成分分析 • 上一篇    下一篇

基于UPLC-Q TOF-MS和HPLC-QQQ-MS技术的赤豆酚类化合物定性、定量分析及其抗氧化能力分析

李文婷,顿 倩,李红艳,邓泽元,张 兵   

  1. 南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047
  • 出版日期:2019-04-25 发布日期:2019-05-05
  • 基金资助:
    国家自然科学基金青年科学基金项目(31701564);江西省自然科学基金项目(20171BAB214035)

Qualitative and Quantitative Analysis of Adzuki Bean Phenolics by Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry and High Performance Liquid Chromatography Coupled with Triple Quadruple Mass Spectrometry and Their Antioxidant Activity

LI Wenting, DUN Qian, LI Hongyan, DENG Zeyuan, ZHANG Bing   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2019-04-25 Published:2019-05-05

摘要: 为深入了解赤豆的营养价值,用80%甲醇溶液提取酚类物质,对提取物中不同存在形态的酚类化合物含量及其抗氧化活性进行测定,并对其80%甲醇提取物中酚类物质进行定性、定量分析。结果表明,赤豆80%甲醇提取物中酚酸含量为3.44 mg/g,黄酮含量为2.50 mg/g,其中3 种存在形式酚类萃取物含量及抗氧化能力不同,糖苷键合态酚酸含量最高,可达1.44 mg/g,游离态黄酮含量最高,可达0.85 mg/g;糖苷键合态亚铁离子还原能力最强,可达15.76 mmol/g,酯键合态1,1-二苯基-2-三硝基苯肼自由基、2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力最强;80%甲醇提取物通过超高效液相色谱串联四极杆飞行时间质谱仪定性分析,初步鉴定出25 种化合物,包括3 种有机酸、6 种酚酸、16 种黄酮;高效液相色谱串联三重四极杆质谱联用仪定量分析可得,芦丁(327.40 μg/g)、儿茶素类(210.70 μg/g)含量丰富。

关键词: 超高效液相色谱串联四极杆飞行时间质谱仪, 高效液相色谱串联三重四极杆质谱联用仪, 赤豆, 酚类, 抗氧化活性

Abstract: Adzuki bean (the seed of Vigna angularis) is a medicinal and edible legume. In order to better understand its nutritional value, phenolics from adzuki bean were extracted with 80% methanol and separated into free, glycoside and esterified forms. The phenolics were qualitatively and quantitatively analyzed. The results showed that the 80% methanol extract contained 3.44 mg/g phenolic acid and 2.50 mg/g flavonoid. The contents of the three forms of phenolics and their antioxidant activities varied significantly, among which the glycosidically bound fraction was found to be the richest in phenolic acid, up to 1.44 mg/g, whereas the free fraction was had the highest flavonoid content of 0.85 mg/g. The glycosidically bound fraction showed the strongest ferric reducing antioxidant power (FRAP) (15.76 mmol/g), while the ester bound fraction presented the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonate sulfonic acid) (ABTS) radical cation scavenging abilities. The 80% methanol extract was analyzed by high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-Q TOF-MS), and a total of 25 compounds including 3 organic acids, 6 phenolic acids and 16 flavonoids were identified. Quantitative analysis by HPLC-QQQ-MS showed that adzuki bean was rich in both rutin (327.40 μg/g) and catechin (210.70 μg/g).

Key words: ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry, high performance liquid chromatography coupled with triple quadruple mass spectrometry, adzuki bean, phenolics, antioxidant activity

中图分类号: