• 食品化学 •

### 环氧基修饰磁性微球固定化磷脂酶A1

1. （合肥工业大学食品科学与工程学院，安徽省农产品精深加工重点实验室，安徽?合肥 230009）
• 出版日期:2019-01-25 发布日期:2019-01-22
• 基金资助:
“十三五”国家重点研发计划重点专项（2016YFD0401401-2）

### Immobilization of Phospholipase A1 into Epoxy-Modified Magnetic Microspheres

BAO Sai, CAO Lili, PANG Min, PAN Lijun, HOU Zhigang, SHUI Longlong, LI Jinhong, JIANG Shaotong*

1. (Key Laboratory for Agriculture Products Processing of Anhui Province, School of Food and Engineering, Hefei University of Technology, Hefei 230009, China)
• Online:2019-01-25 Published:2019-01-22

Abstract: The epoxy-modified Fe3O4 magnetic microspheres prepared in our laboratory were used as the carrier to immobilize phospholipase A1 (PLA1). Response surface methodology was used to optimize the immobilization conditions. Furthermore, the properties of the immobilized enzyme were studied. The results showed that the optimal immobilization conditions were determined as follows: buffer pH 4.0, reaction time 1.9 h and enzyme dosage 3.6 mL/g. The activity of the immobilized enzyme obtained under these conditions was 3 675 U/g with an immobilization efficiency of 61.1%. Compared with the free enzyme, the optimal reaction pH was shifted to alkaline pH of 1.0 and the optimal reaction temperature of the immobilized enzyme was increased by 5?℃. The storage stability of the immobilized enzyme was increased as well. And the immobilized enzyme retained about 81.1% of the initial activity after the 8th use for rapeseed oil degumming. Moreover, modern analytical methods such as X-ray diffraction (XRD), attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the carrier. The results showed that the nano-sized microspheres were successfully prepared with epoxy-modified surfaces.