食品科学 ›› 2019, Vol. 40 ›› Issue (1): 240-248.doi: 10.7506/spkx1002-6630-20180521-296

• 包装贮运 • 上一篇    下一篇

外源钙和钙调素拮抗剂对冷藏桃果实耐冷性的影响

凌 晨,谢 兵,洪羽婕,王 莉,金 鹏*,郑永华   

  1. 南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    国家自然科学基金面上项目(31671901);南京农业大学SRT计划项目(1618C06)

Effects of Exogenous Calcium and Calmodulin Antagonist Treatments on Chilling Tolerance of Cold-Stored Peach Fruit

LING Chen, XIE Bing, HONG Yujie, WANG Li, JIN Peng*, ZHENG Yonghua   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 以‘白凤’水蜜桃为试材,分别用外源钙(CaCl2)和钙调素拮抗剂三氟拉嗪(trifluoperazin,TFP)浸泡桃果实,以蒸馏水为对照,研究其对冷藏桃果实抗冷性的影响。结果表明:与对照组相比,CaCl2处理能有效减轻桃果实果心褐变程度,缓解冷害症状,降低相对电导率,降低丙二醛(malondialdehyde,MDA)、H2O2含量,降低超氧阴离子自由基(O2 - ·)产生速率以及脯氨酸脱氢酶(proline dehydrogenase,PDH)活力,提高活性氧代谢相关酶(超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)、Δ1-吡咯啉-5-羧酸合成酶(Δ1-pyrroline-5-carboxylate synthetase,P5CS)、鸟氨酸氨基转移酶(ornithine δ-aminotransferase peroxidase,OAT)、谷胱甘肽还原酶(glutathione reductase,GR))活力;另外,CaCl2处理后的桃果实表现为较高的脯氨酸、VC和谷胱甘肽(glutathione,GSH)含量;而TFP处理后的桃果实冷藏特性与CaCl2处理组相反。这说明钙-钙调素(Ca2+-CaM)复合体参与了采后桃果实抗冷性调控,Ca2+浓度的增加能维持桃果实活性氧代谢平衡,减少低温胁迫下膜脂过氧化与损伤,增加渗透调节物质脯氨酸的积累,从而减少桃果实冷藏期间冷害的发生,保持果实营养品质,延长果实贮藏时间。

关键词: 钙, 三氟拉嗪, 桃果实, 冷害, 耐冷性

Abstract: The effects of calcium (CaCl2) and calmodulin antagonist trifluoperazin (TFP) dipping treatments on chilling tolerance of postharvest peach fruit (Prunus persica Batsch ‘Baifeng’) during cold storage were investigated with distilled water as control. The results showed that CaCl2 treatment could significantly reduce internal browning of peach fruit during cold storage, mitigate chilling injury, decrease relative electric conductivity and malonaldehyde (MDA) content, reduce hydrogen peroxide (H2O2) content and superoxide anion radical (O2 -·) production rate, and the activity of proline dehydrogenase (PDH). In addition, CaCl2 treatment improved the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), Δ1-pyrroline-5-carboxylate synthetase (P5CS), ornithine δ-aminotransferase peroxidase (OAT) and glutathione reductase (GR). High levels of proline, vitamin C and glutathione (GSH) were also observed in peach fruit treated with CaCl2. However, the opposite effect was observed with TFP treatment. These findings suggested that the Ca2+-CaM complex was involved in the regulation of cold tolerance in postharvest peach fruit and that CaCl2 treatment could maintain reactive oxygen metabolism balance, alleviate lipid peroxidation and membrane damage, and increase the accumulation of the osmoregulator proline, thereby reducing chilling injury, maintaining fruit quality and extending the storage life of peach fruits during cold storage.

Key words: calcium, trifluoperazin, peach fruit, chilling injury, chilling tolerance

中图分类号: