可溶性大豆多糖改善左旋肉碱诱导的小鼠小肠首过代谢

doi:10.7506/spkx1002-6630-20180620-388

摘要/Abstract

摘要： 目的：研究大豆可溶性多糖（soybean soluble polysaccharides，SSPS）对左旋肉碱喂养小鼠的小肠代谢、氧化应激和炎症相关蛋白表达的影响。方法：24 只雄性昆明小鼠随机分成正常组、左旋肉碱组、SSPS组3 个实验组。连续饲喂56 d后处死小鼠，测定小鼠小肠内P-糖蛋白（P-glycoprotein，P-gp）、多药耐药性蛋白（multidrug resistanceproteins，MRP）1、MRP3、白细胞介素（interleukin，IL）-1、IL-6、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）质量浓度和尿苷二磷酸葡萄糖醛酸基转移酶（uridine diphosphate glucuronosyltransferase，UGT）活力、硫酸基转移酶（sulfotransferase，SULT）活力、丙二醛（malondialdehyde，MDA）浓度等的变化。结果：与正常组相比，左旋肉碱组小鼠小肠具有更高的P-gp和MRP3质量浓度（P＜0.05）；而SSPS可使左旋肉碱喂养小鼠小肠内P-gp质量浓度显著降低（P＜0.05），且使MRP3质量浓度轻微下降，表明SSPS可有效抑制左旋肉碱诱导的首过代谢。此外，SSPS与左旋肉碱联合处理可有效避免小鼠小肠组织MDA浓度的增加、超氧化物歧化酶活力的下降和·OH清除能力的减弱，预示SSPS可抑制长期摄入左旋肉碱诱导的氧化应激。然而，所有小鼠小肠组织的SULT和UGT活力以及IL-1、IL-6、TNF-α质量浓度没有显著变化（P＞0.05），表明过量左旋肉碱和SSPS处理不会影响II相代谢，也不会导致炎症反应。此外，代谢组学分析表明，SSPS可抑制长期过量摄入左旋肉碱导致的能量代谢及脂质代谢紊乱。结论：SSPS可以通过调控氧化应激和代谢紊乱而有效预防左旋肉碱诱导的小鼠小肠首过代谢。

关键词: 大豆可溶性多糖, 左旋肉碱, 首过代谢, 氧化应激, 炎症

Abstract: Objective: To study the effect of soluble soybean polysaccharides (SSPS) on intestinal metabolism, oxidative stress and inflammation-related proteins in mice fed L-carnitine. Methods: A total of 24 male Kunming mice were randomly divided into three groups: normal group, L-carnitine group and SSPS group. At the end of the 56-day feeding period, all mice were sacrificed to assess the changes in the levels of P-glycoprotein (P-gp), multidrug resistance protein (MRP) 1, MRP3, interleukin (IL)-1, IL-6, tumor necrosis factor-α (TNF-α), uridine diphosphateglucuronosyltransferase (UGT), sulfotransferase (SULT), and malondialdehyde (MDA) in the small intestine. Results: The concentrations of P-gp and MRP3 in the small intestine homogenate supernatant of mice in the L-carnitine group were significantly higher than that of the normal group (P < 0.05). However, SSPS significantly decreased P-gp concentration (P < 0.05), and slightly decreased MRP3 concentration in the small intestine of L-carnitine-fed mice, suggesting that SSPS could inhibit L-carnitine-induced first pass metabolism. Additionally, co-treatment of SSPS and L-carnitine on mice effectively prevented the increase of MDA content, the decrease of superoxide dismutase (SOD) activity, and the weakening of hydroxyl radical-scavenging capacity, indicating that SSPS could inhibit L-carnitine-induced oxidative stress. However, the activities of SULT and UGT, and the concentrations of IL-1, IL-6 and TNF-α in the small intestine did not differ significantly among all groups of mice (P > 0.05), suggesting that excessive L-carnitine and SSPS treatment did not affect the II phase metabolism or lead to inflammation. Furthermore, metabonomic analysis showed that SSPS could inhibit the disorders of energy metabolism and lipid metabolism caused by long-term excessive intake of L-carnitine. Conclusion: The first pass metabolism induced by L-carnitine can be effectively prevented by SSPS via controlling oxidative stress and metabolic disorders.

Key words: soluble soybean polysaccharide, L-carnitine, first pass metabolism, oxidative stress, inflammation

中图分类号:

TS201.4

李文峰，陶雯，陈露红，郑俏然，周凤，谭飔，邢洁. 可溶性大豆多糖改善左旋肉碱诱导的小鼠小肠首过代谢[J]. 食品科学, 2019, 40(13): 187-194.

LI Wenfeng, TAO Wen, CHEN Luhong, ZHENG Qiaoran, ZHOU Feng, TAN Si, XING Jie. Soluble Soybean Polysaccharides Ameliorate L-Carnitine-Induced First-Pass Metabolism in the Small Intestine of Mice[J]. FOOD SCIENCE, 2019, 40(13): 187-194.

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可溶性大豆多糖改善左旋肉碱诱导的小鼠小肠首过代谢

Soluble Soybean Polysaccharides Ameliorate L-Carnitine-Induced First-Pass Metabolism in the Small Intestine of Mice

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