食品科学 ›› 2018, Vol. 39 ›› Issue (8): 218-223.doi: 10.7506/spkx1002-6630-201808034

• 安全检测 • 上一篇    下一篇

石墨烯电化学传感器法快速测定植物油中的苯并(a)芘

冯亚净,李书国*   

  1. (河北科技大学生物科学与工程学院,河北?石家庄 050018)
  • 出版日期:2018-04-25 发布日期:2018-04-17
  • 基金资助:
    国家自然科学基金面上项目(20876165);河北省食品药品安全科技计划项目(ZD2014011)

Graphene-Based Electrochemical Sensor for Rapid Determination of Benzo(a)Pyrene in Vegetable Oils

FENG Yajing, LI Shuguo*   

  1. (College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China)
  • Online:2018-04-25 Published:2018-04-17

摘要: 利用新型纳米材料石墨烯与聚合物材料壳聚糖构建纳米电化学传感器,研究苯并(a)芘(benzo(a)pyrene,BaP)的电化学性质,并建立一种植物油中BaP的快速灵敏检测方法。BaP在该纳米电化学传感器上的循环伏安图表明在1.0?V处出现了一个形状良好的氧化峰,无还原峰,为一种不可逆的氧化还原过程;优化纳米电化学传感器法快速测定植物油中BaP的实验条件,结果表明:壳聚糖-石墨烯(1∶2,V/V)混合溶液作为修饰液,修饰量5?μL、电解质LiClO4浓度0.2?mol/L、硫酸浓度0.1?mol/L、富集时间15?min,在此条件下,BaP的氧化还原峰电流与其浓度在0~100?nmol/L之间具有良好的线性关系,校正曲线方程为:Ip=0.116?2CBaP+22.926?2(R2=0.997?8),其检测限为0.103?nmol/L(RSN=3)。利用该法对香油样品中的BaP进行检测,其加标回收率为98.51%~100.57%,检测结果与高效液相色谱法基本一致,并且具有良好的稳定性和重复性,样品预处理简单、检测时间短、速度快、成本低。

关键词: 苯并(a)芘(BaP), 电化学传感器, 食品安全, 植物油

Abstract: An electrochemical sensor was constructed by immobilizing graphene as a new nanomaterial and chitosan on glassy carbon electrodes. The electrochemical properties of benzo(a)pyrene were investigated? and a rapid and sensitive method for the detection of benzo(a)pyrene in vegetable oils was established. A well-defined oxidation peak was observed at 1.0 V according to the cyclic voltammogram of benzo(a)pyrene at the nano electrochemical sensor and no reduction peak appeared, indicating that this is an irreversible redox process. The experimental conditions for electrochemical detection of benzo(a)pyrene in vegetable oils were optimized as follows: 5 μL of a mixed solution of chitosan-graphene (1:2, V/V) was used as modified solution; the concentration of LiClO4 as electrolyte was 0.2 mol/L; sulfuric acid concentration was 0.1 mol/L; and the enrichment time was 15 min. Under these optimal conditions, there was a good linear relationship between the redox peak current of benzo(a)pyrene and its concentration in the range of 0–100 nmol/L ( Ip = 0.116 2 CBaP + 22.926 2, R2 = 0.997 8), and the limit of detection (LOD) was 0.103 nmol/L (RSN = 3). The recoveries of benzo(a)pyrene in spiked sesame oil were in the range of 98.51%–100.57%. The results well agreed with those obtained by high performance liquid chromatography. The proposed method had good stability and repeatability and was characterized by simple sample pretreatment, short detection time, rapidity and low cost.

Key words: benzo(a)pyrene, electrochemical sensor, food safety, vegetable oils

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